静脉注射人胎盘间充质干细胞对油酸型急性肺损伤炎症调节的作用  

Regulatory effects of human placenta derived mesenchymal stem cells on the treatment for OA-induced acute lung injury

作　　者：崔晴晴[1] 邢朝品[1] 林新梅[1] 张新顺[1] 段培锋[1] 李高峰[2] CUI Qingqing;XING Chaopin;LIN Xinmei;ZHANG Xinshun;DUAN Peifeng;LI Gaofeng(Department of Pediatrics,Binzhou Medical University Hospital,Binzhou 256603,Shandong,P.R.China;Department of Ultrasound,Binzhou Medical University Hospital)

机构地区：[1]滨州医学院附属医院儿童消化与肾病科,山东滨州256603 [2]滨州医学院附属医院超声医学科

出　　处：《滨州医学院学报》2020年第3期169-171,189,共4页Journal of Binzhou Medical University

基　　金：滨州医学院科研计划(BY2015KJ20)。

摘　　要：目的探讨静脉注射人胎盘间充质干细胞(hPMSCs)对油酸型急性肺损伤(ALI)的治疗作用及可能相关机制。方法大鼠36只随机分为正常对照组、肺损伤组、治疗组采用油酸舌下静脉注射的方法制备ALI模型。造模成功后给予相应处理后24 h留取标本,观察不同组别的病理组织形态、肺组织的湿干质量比(W/D)、肺泡灌洗液中性粒细胞比例、血清白介素10(IL-10)、白介素8(IL-8)水平。结果肺组织病理学观察,正常组肺泡组织构正常,肺损伤组肺组织出现典型的ALI改变,细胞移植组损伤程度较轻。与对照组比较,损伤组和移植组支气管肺泡灌洗液中性粒细胞数目明显升高(P<0.05或<0.01),损伤组和移植组支气管肺泡灌洗液W/D比也升高(P<0.05)。与肺损伤组比较,移植组支气管肺泡灌洗液中性粒细胞数目、W/D比均降低(P<0.05)。与对照组比较,损伤组和移植组血浆及肺组织IL-8、IL-10水平明显升高(P<0.05或<0.01);与损伤组比较,移植组IL-8的水平明显降低(P<0.05或<0.01)。结论hPMSCs移植治疗ALI可减轻致炎因子水平,提高抗炎因子水平,稳定体内环境,避免炎症风暴的发生,为临床治疗急危重症提供有效的治疗手段。Objective To investigate the therapeutic effect of human placenta mesenchymal stem cells(hPMSCs)on OA-induced acute lung injury and its possible mechanism.Methods Thirty-six SD rats were divided into the control group,the lung injury group and the treatment group.Acute lung injury rat model was prepared by sublingual intravenous injection of oleic acid.After the successful modeling,the samples were kept for 24 h after the corresponding treatment,and the lung tissue morphology,the ratio of wet to dry mass of lung tissue(W/D),the proportion of neutrophils in alveolar lavage fluid and the serum IL-10,IL-8 level were detected.Results Pulmonary histopathology showed that normal alveolar structure was normal in the normal group,typical ALI changes were observed in the lung tissue of the lung injury group,and the degree of injury in the cell transplantation group was relatively mild.Compared with the control group,the number of neutrophils in bronchoalveolar lavage fluid was significantly increased in the injury group and the transplantation group(P<0.05 or<0.01),and the wet/dry ratio of bronchoalveolar lavage solution was also increased in the injury group and the transplantation group(P<0.05).Compared with the lung injury group,the number of neutrophils and the wet/dry ratio of bronchoalveolar lavage fluid in the transplantation group were all decreased(P<0.05).Plasma and lung tissue levels of IL-8 and IL-10 in the injury group and the transplantation group were significantly increased,compared with the control group(P<0.05 or<0.01);IL-8 levels in the transplant group were significantly reduced compared with the injury group(P<0.05 or<0.01).Conclusion Placental mesenchymal stem cell transplantation in the treatment of acute lung injury can reduce the level of inflammatory factors,improve the level of anti-inflammatory factors,stabilize the internal environment,avoid the occurrence of inflammatory storms,and provide an effective treatment method for the clinical treatment of critical patients.

关 键 词：急性肺损伤 间充质干细胞 白介素10 白介素8 细胞移植 

分 类 号：R563.1[医药卫生—呼吸系统]