鲜食甜玉米南方锈病抗性QTL定位分析  

QTL mapping for Southern rust resistance in fresh sweet corn

作　　者：张金钰 蒲浩杰 刘鹏飞 陈青春 张姿丽 蒋锋 ZHANG Jinyu;PU Haojie;LIU Pengfei;CHEN Qingchun;ZHANG Zili;JIANG Feng(College of Agronomy and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Yunfu Yuncheng District Agricultural Rural and Water Bureau,Yunfu 527300,China)

机构地区：[1]仲恺农业工程学院农业与生物学院,广州510225 [2]广东省云浮市云城区农业农村和水务局,广东云浮527300

出　　处：《中国农业大学学报》2020年第4期82-88,共7页Journal of China Agricultural University

基　　金：广东省鲜食玉米遗传育种工程中心(粤科产学研[2016]176号);广东省农业厅种业提升项目(粤财农[2014]492号);广东省重点领域研发计划(2018B020202013);广州国家现代农业产业科技创新中心创建项目(2018KCZX02)。

摘　　要：为挖掘新的抗南方锈病基因资源,本研究以甜玉米组合M5×M114的216个F2单株为遗传作图群体,应用BSA方法从500对SSR引物中筛选出2对在F2代抗病和感病DNA池间具有多态性的引物,分别位于4和9号染色体上;在4和9号染色体上重新设计100对SSR引物,构建了包含33个标记位点总长为241.2cM的连锁遗传图,各个标记间的平均距离为7.53cM。结合F2单株对南方锈病的抗性表现,用复合区间作图法在4和9号染色体上共检测到7个显著的南方锈病抗性QTLs,其中:4个QTLs位于4号染色体上,可解释12.1%、7.8%、18.2%和14.9%表型变异;3个位于9号染色体上,分别解释17.0%、13.3%与19.2%的表型变异。研究结果可为抗南方锈病的精细定位、主效基因克隆和抗南方锈病鲜食甜玉米品种选育提供理论依据。To explore new genetic resources to Southern rust in this study,216 F2 individuals of sweet corn combination M5×M114 were used as genetic mapping population to scan resistant QTL of maize Southern rust with the method of Bulked Segregant Analysis.The results showed that:Over 500 SSR markers screened,two markers located on chromosomes 4 and 9 showed polymorphic between resistant and susceptible DNA pools.A linkage genetic map containing 33 marker loci with a total length of 241.2 cM was constructed by redesigning 100 pairs of SSR primers on chromosomes 4 and 9,and the average distance between individual markers was 7.53 cM.Combined with the resistance index of 216 F2 families,a total of 7 significant Southern rust resistance QTLs were detected on chromosomes 4 and 9 by composite interval mapping.Four QTLs were located on chromosome 4 and respectively explained 12.1%,7.8%,18.2%and 14.9%of phenotypic variation.And 3 significant QTLs were located on chromosome 9,which explained 17.0%,13.3%and 19.2%of the phenotypic variation,respectively.This study provided a theoretical basis for QTL fine mapping of Southern rust resistance,cloning of major genes and breeding Southern rust resistant varieties for maize.

关 键 词：甜玉米 南方锈病 群体分离分析 QTL作图 

分 类 号：S513[农业科学—作物学]