细胞3D培养技术及其在水产养殖上的应用  

3D cell culture technology and its application in aquaculture

作　　者：唐旖 蔡灵 杨明[2] TANG Yi;CAI Ling;YANG Ming(Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China;School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China)

机构地区：[1]自然资源部第三海洋研究所,福建厦门361005 [2]上海大学环境与化学工程学院,上海200444

出　　处：《渔业研究》2020年第3期288-292,共5页Journal of Fisheries Research

基　　金：自然资源部第三海洋研究所基本科研项目[典型环境雌激素污染物对海洋鱼类免疫毒性及生态风险评估方法研究(海三科2017019)].

摘　　要：细胞培养技术自19世纪研究成功以来,已在遗传学、免疫学、实验医学和肿瘤学等各个学科作出了重要贡献,成为相关领域重要的核心和基础技术之一。细胞的三维(3D)培养是在传统的细胞培养技术上发展起来的一种新的培养方法,可以更好地模拟细胞在生物体内的生长环境和状况,受到越来越多研究者的重视。水产动物的细胞培养是从20世纪60年代以后发展起来的,最初用于病毒的分离和纯化,对水产动物的疾病防治具有重要意义。本文在对3D细胞培养技术的特点、发展历程总结的基础上,对3D细胞培养技术在水产养殖上的应用进行简介和展望。Cell culture technology has made important contributions since 19th century,in various disciplines such as genetics,immunology,experimental medicine,and oncology.Cell culture of aquatic animals began in the 1960s.It was originally used for the isolation and purification of viruses and is of great significance for the prevention and treatment of diseases in aquatic animals.Three-dimensional(3D)culture of cells is a new culture method developed based on traditional cell culture technology,which can better simulate the growth environment and condition of cells in living organisms,and has gained more and more attention.Based on the summary of the characteristics and development of 3D cell culture technology,this paper briefly introduced and prospected the application of 3D cell culture technology in aquaculture.

关 键 词：3D细胞培养 发展历程 水产养殖 

分 类 号：Q2-33[生物学—细胞生物学]