GRP78在不同亚型宫颈癌细胞株中表达情况及对顺铂敏感性影响  

Expression of GRP78 and Its Effect on Cisplatin Sensitivity in Different Subtypes of Cervical Cancer Cells

作　　者：张冉浠 周莉[2] 陆安伟 秦娟 Zhang Ranxi;Zhou Li;Lu Anwei;Qin Juan(College of Clinical Medicine,Guizhou Medical University,Guiyang 550004,Guizhou,China;Department of Gynecologic Oncology,Guiyang Maternal and Child Health Hospital&Guiyang Children’s Hospital,Guiyang 550004,Guizhou,China;Department of Gynecologic Oncology,Shenzhen Hospital of Southern Medical University,Shenzhen 518000,China)

机构地区：[1]贵州医科大学临床医学院,贵阳550004 [2]贵阳市妇幼保健院妇科肿瘤科,贵阳550004 [3]南方医科大学深圳医院妇科,广东深圳518000

出　　处：《肿瘤预防与治疗》2020年第5期389-394,共6页Journal of Cancer Control And Treatment

基　　金：国家自然科学基金(编号:81860328);贵州省科技计划项目(编号:黔科合平台人才[2016]5603);贵州省科学技术基金项目(编号:黔科合J字[2013]2020号);贵阳市科技计划项目(编号:筑科合同[20161001]14号)。

摘　　要：目的:研究不同亚型宫颈癌细胞株中葡萄糖调节蛋白78(glucose regulated protein 78,GRP78)表达情况,以及GRP78表达水平对宫颈癌细胞顺铂敏感性的影响。方法:采用免疫组织化学法检测人乳头瘤病毒(human papillomavirus,HPV) 16(+)SiHa、HPV18(+)HeLa以及HPV(-)C33a宫颈癌细胞中的GRP78表达,MMT、Western blot法检测以上3种细胞在不同浓度顺铂、衣霉素+顺铂处理后的细胞抑制率及GRP78表达水平,采用单因素方差分析,分析相同浓度下单用顺铂、衣霉素+顺铂联合处理后3种细胞的细胞抑制率及GRP78蛋白表达的差异。结果:SiHa、HeLa细胞GRP78阳性表达水平明显高于C33a细胞,SiHa细胞GRP78阳性表达水平最高(P<0.01)。随着顺铂浓度增加,3种宫颈癌细胞抑制率增加呈浓度依赖性,且HeLa细胞抑制率最高(P<0.05)。与单独顺铂组相比,衣霉素联合顺铂药物处理组中HeLa及SiHa细胞抑制率显著升高(P<0.05),并且GRP78表达水平也明显升高(P<0.01),且各细胞株中GRP78表达水平均随顺铂浓度增加而增加。结论:GRP78表达水平与宫颈癌细胞亚型有关,SiHa、HeLa细胞对顺铂敏感性高于C33a细胞。衣霉素导致GRP78明显高表达,增加了SiHa、HeLa细胞对顺铂的敏感性。Objective:To study the expression of glucose-regulated protein 78(GRP78)in different subtypes of cervical cancer cells and the effect of GRP78 expression on the sensitivity of cervical cancer cells to cisplatin.Methods:Immunohis-tochemistry was used to detect the expression of three cervical cancer cells,GRP78 in human papillomavirus(HPV)16-posi-tive SiHa,HPV18 positive-HeLa and HPV-negtive C33a.Methyl thiazolyl tetrazolium and Western blot were used to detect the inhibition rate and GRP78 level of the above cells treated with different concentration of cisplatin and tunicamycin plus cisplatin.One-way ANOVA was used to analyze the differences in inhibition rate and GRP78 level in three kinds of cells treated with cisplatin and tunicamycin+cisplatin at the same concentration.Results:The GRP78 level in SiHa and HeLa were significantly higher than that in C33a,and the expression of GRP78 in HeLa was the highest(P<0.01).As the con-centration of Cisplatin increased,the inhibition rate increased in a concentration-dependent manner,and the inhibition rate of HeLa was the highest(P<0.05).Compared with the Cisplatin group,the inhibition rate of HeLa and SiHa and the GRP78 level in the tunicamycin plus cisplatin group were significantly higher,and the GRP78 level in all cell lines increased as the concentration of cisplatin increased.Concusion:The GRP78 level is related to the subtypes of cervical cancer cells.The sensitivity of SiHa and HeLa to cisplatin is higher than that of C33a.Tunicamycin induces higher expression of GRP78,which increases the sensitivity of SiHa and HeLa to cisplatin.

关 键 词：宫颈癌 GRP78 内质网应激 顺铂 

分 类 号：R737.33[医药卫生—肿瘤] R730.53[医药卫生—临床医学]