川芎抗偏头痛作用的有效部位筛选及指纹图谱研究  

Screening of Active Fraction from Chuanxiong for Anti-Migraine Function and Its Fingerprint

作　　者：陈玲[1] 马俊 唐艺 何郡 邓薇[1] 蒋桂华[1] 崔建蓉[2] Chen Ling;Ma Jun;Tang Yil;He Jun;Deng Wei;Jiang Guihua;Cui Jianrong(Chengdu University of Traditional Chinese Medicine State Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese Medicine Resources,Chengdu 611137;Pharmacy department,Chengdu Seventh Peopled Hospital,Chengdu 610041)

机构地区：[1]成都中医药大学,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137 [2]成都市第七人民医院药剂科,成都610041

出　　处：《中药药理与临床》2020年第1期80-85,共6页Pharmacology and Clinics of Chinese Materia Medica

基　　金：科技基础性工作专项-常用道地药材及其产区的特征、标准及数字化(2015FY111500-140);"杏林学者"学科人才科研提升学术骨干项目(XSGG-2019023);"杏林学者"学科人才科研提升青年学者项目(QNXZ2019024);四川省科技厅扶贫项目(2018NFP0112)。

摘　　要：目的:确定川芎对偏头痛模型大鼠镇痛作用的有效部位,并建立该有效部位的HPLC指纹图谱,为其质量控制提供可靠有效的方法。方法:采用皮下注射硝酸甘油建立大鼠偏头痛模型,比较川芎各有效部位的镇痛效果,并通过HPLC对各有效部位的化学成分进行对比分析,确定川芎治疗偏头痛的主要有效部位。采用GS-120-5-C18(250mm×4. 6mm,5μm)色谱柱;以甲醇-水为流动相梯度洗脱;流速1. 0 m L/min;柱温25℃;检测波长280 nm;进样量20μL。进行不同产地川芎乙酸乙酯部位的指纹图谱研究,并对所得指纹图谱进行相似度评价和主成分分析。结果:川芎各有效部位化学成分的种类和含量存在差异。石油醚部位主要为脂溶性成分,二氯甲烷部位、乙酸乙酯部位以及正丁醇部位既有脂溶性成分,也有水溶性成分,但含量有所差异。结合药效学试验结果分析,川芎治疗偏头痛的主要有效部位为乙酸乙酯有效部位;24批川芎的乙酸乙酯有效部位共确立了8个共有峰,建立了川芎乙酸乙酯部位的HPLC对照指纹图谱,通过结合保留时间和紫外光谱,指认了洋川芎内酯I、洋川芎内酯H、洋川芎内酯A、藁本内酯4个特征峰。24批川芎乙酸乙酯有效部位的相似度在0. 967~1. 000之间。对8个共有峰进行主成分分析,其综合评判得分在-0. 584~1. 145之间。结论:川芎乙酸乙酯部位是抗偏头痛作用的主要有效部位,所建立的指纹图谱具有良好的精密度、重复性和稳定性,可以为川芎乙酸乙酯部位的质量控制提供全面的信息。Objective: To screen the active fraction from Chuanxiong in the treatment of migraine and to establish an HPLC fingerprint of the active fractions,in order to provide effective method for the quality control. Methods: Nitroglycerin was subcutaneously injected to establish the ratmodel with migraine. The analgesic effect of different fractions were compared,also the chemical compounds of different active fractions were compared by HPLC,in order to find the main active fraction in the treatment of migraine. HPLC analysis was carried out on a GS-120-5-C18( 250 ㎜ × 4. 6 ㎜,5 μm) column with the gradient elution of methanol and water. The flow rate,column temperature,detection wavelength and injection volume were 1. 0 m L/min,25 ℃,280 nm and 20 μL,respectively. The similarity evaluation and principal component analysis were used to analyze HPLC fingerprint of active fractions from Chuanxiong from different origins. Results: There were differences in the species and contents of the chemical constituents of different active fractions from Chuanxiong. The main components from petroleum ether extraction were fat-soluble components. The main components from dichloromethane extraction,ethyl acetate extraction and N-butanol extraction were both fat-soluble and water-soluble components,while their contents were different. Combined with the pharmacological results,the ethyl acetate extraction was the active fraction from Chuanxiong in the treatment of migraine. HPLC fingerprint of the active fraction was established with 8 common peaks by determining 24 batches of samples. 4 characteristic peaks,including senkyunolide I,senkyunolide H,senkyunolide A and ligustilide were confirmed by comparing their retention time and UV spectrum with standard reference substance. The similarities of 24 batches of ethyl acetate extraction from Chuanxiong were between 0. 967 ~ 1. 000. Comprehensive evaluation scores of 24 batches of ethyl acetate extraction from Chuanxiong were between-0. 584 ~ 1. 145. Conclusion: Ethyl acetate extractio

关 键 词：川芎 偏头痛 乙酸乙酯有效部位 HPLC指纹图谱 

分 类 号：R285.5[医药卫生—中药学]