城市和农村段河流沉积物中抗性基因分布规律比较分析——以嘉陵江支流西河为例  

Comparative Analysis on the Distribution Pattern of Antibiotic Resistance Genes in River Sediments between Rural and Urban Sections——A Case Study of Xihe Stream of Jialing River

作　　者：黄丽萍 刘燕 何燕 彭超 钱永明 路璐 HUANG Liping;LIU Yan;HE Yan;PENG Chao;QIAN Yongming;LU Lu(College of Environmental Science and Engineering,Nanchong Sichuan 637009,China;College of Life Sciences,China West Normal University,Nanchong Sichuan 637009,China)

机构地区：[1]西华师范大学环境科学与工程学院,四川南充637009 [2]西华师范大学生命科学学院,四川南充637009

出　　处：《西华师范大学学报（自然科学版）》2020年第2期125-133,共9页Journal of China West Normal University(Natural Sciences)

基　　金：国家自然科学基金项目(41606142);西华师范大学英才科研基金项目(17YC140)。

摘　　要：抗生素的大量使用导致环境中微生物耐药性以及抗性基因的产生和传播,严重威胁人类的健康。本文以嘉陵江支流西河农村段和城市段的沉积物为研究对象,采用80对抗性基因引物进行PCR检测,以期揭示2个河段中氨基糖苷类、四环素类和磺胺类等三类抗生素抗性基因的多样性和相对丰度的差异。结果表明,城市段的抗性基因多样性(75种、检出率为93.8%)高于农村段(63种,检出率为78.8%),其中,氨基糖苷类、磺胺类、四环素类抗性基因在城市段的检出率分别为97.2%、71.4%、91.9%,而农村段的检出率分别为69.4%、57.1%、94.6%。城市段的主导氨基糖苷抗性基因为aacA/aphD、aadA2-03、spcN-02,农村段为aadA1和aadA2-03;城市段的主导磺胺抗性基因为sul2,农村段为dfrA1;城市段的主导四环素抗性基因为tet(36)-2、tetM-01、tetM-02、tetG-02,农村段为tetM-01、tetPB-05、tetL-01。综上,西河农村段和城市段河流沉积物中的抗性基因组成有显著差异,这可能与农村和城市的岸源污染物组成差异有关,该研究为探究城市和农村源污染对河流抗性基因的贡献有重要借鉴意义。The extensive use of antibiotics has led to the emergence and spread of microbial resistance and antibiotic resistance genes(ARGs)in the microorganism,posing a threat to the ecological environment and human health.Taking the sediments from the rural and urban sections of Xihe Stream in Jialing River as the research object,this paper reveals the diversity and relative abundance of aminoglycosides-,tetracycline-and sulfonamides-resistance genes through PRC invetigation of 80 pairs of antibiotic resistance genes primer.The results show that the diversity of ARGs(75 subtypes,detection rate of 93.8%)in the urban section is significantly higher than that in the rural section(63 types,detection rate of 78.8%).Among them,the detection rates of aminoglycosides-,sulfonamides-and tetracycline-resistance genes in urban segments are 97.2%,71.4%,and 91.9%respectively,whereas in rural segments are 69.4%,57.1%,and 94.6%.The dominant aminoglycosides resistance genes are aacA/aphD,aad A2-03,spcN-02 in urban segment while in rural segments are aadA1 and aadA2-03.The dominant sulfonamides resistance gene is sul2 in urban segment and dfrA1 in rural segment.The dominant tetracycline resistance genes are tet(36)-2,tetM-01,tetM-02 and tetG-02 in urban segment while in rural segments are tetM-01,tetPB-05 and tetL-01.In summary,there are significant differences in the composition of sediment ARGs between the rural and urban sections of Xihe Stream,which is possibly related to the different composition of shore-source pollutants.This study is of referencial significance for exploring the impact of urban and rural source pollution on river resistance genes.

关 键 词：抗生素抗性基因 聚合酶链式反应 河流沉积物 城市污染源 农村污染源 

分 类 号：X522[环境科学与工程—环境工程]