基于限制性内切酶简化基因组测序的两种主要技术  

The Two Major Technologies of Sequencing Based on Simplified Genome by Restriction Enzyme Digestion

作　　者：张羽[1] 周婉莹 孙旺 Zhang Yu;Zhou Wanying;Sun Wang(School of Biological Sciences and Engineering,Shaanxi University of Technology,Hanzhong,723000)

机构地区：[1]陕西理工大学生物科学与工程学院,汉中723000

出　　处：《分子植物育种》2020年第11期3562-3570,共9页Molecular Plant Breeding

基　　金：陕西省科技厅农业科技攻关项目(2013K02-10-01);陕西省科技厅农业科技创新项目(NKC01-01);陕西省科技统筹创新工程计划(2011KTCG 02-01)共同资助。

摘　　要：随着二代测序技术的发展,其在基因分型、连锁图谱构建、QTLs定位、系统进化、生物多样性、全基因组关联等生物研究领域中的应用越来越广泛。为了降低测序成本,采用一套简化测序的数据已经能满足大多数生物学研究。本研究主要从基于限制性内切酶简化基因组测序的2种主要技术(RAD-seq和GBS)进行介绍,从其原理、测序群体选择、限制性内切酶筛选、测序后数据分析等方面予以阐述。目前,GBS技术以其过程简单且使用甲基化敏感酶回避了基因组重复区域成为了简化测序的主要技术,尤其适合大样本量的研究,可快速鉴定出高密度的变异,加快生物研究的进程。With the development of the next-generation sequencing technology,it is more and more widely used in the field of biological research such as genotyping,linkage map construction,QTLs localization,phylogeny,biodiversity,and genome-wide association.To reduce the cost of sequencing,a set of data that simplifies sequencing has been able to satisfy most biological studies.This study mainly introduces two main technologies(RAD-seq and GBS)based on restriction enzyme simplified genome sequencing,and explain it from the principle,sequencing population selection,restriction enzyme screening,post-sequencing data analysis,etc..At present,GBS technology has become a main technology to simplify sequencing for simple process and using methylation sensitive enzymes avoiding the genomic repeat region.It is especially suitable for large sample size research,which can quickly identify high-density mutations and accelerate biological research.

关 键 词：限制性内切酶 简化基因组 二代测序 数据分析 

分 类 号：Q78[生物学—分子生物学]