小麦-华山新麦草易位系H1684细胞学鉴定及抗条锈性遗传分析  

Cytological Identification and Resistance Inheritance in Wheat-Psathyrostachys huashanica Translocation Line H1684

作　　者：李寒 卢涛 黄文娣 杨扬 詹闯 马东方 尹军良[1,2] Li Han;Lu Tao;Huang Wendi;Yang Yang;Zhan Chuang;Ma Dongfang;Yin Junliang(Hubei Collaborative lnnovation Center for Grain Industry,Hubei Engineering Research Centerfor Pest Forewarming and Management,Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,College of Agriculure,Yangtze University,Jingzhou,434025;College of Plant Protection,Northwest A&F University,Yangling,712100)

机构地区：[1]长江大学农学院,主要粮食作物产业化湖北省协同创新中心,湖北省病虫害预警与调控工程技术研究中心,湿地生态与农业利用教育部工程研究中心,荆州434025 [2]西北农林科技大学植物保护学院,杨陵712100

出　　处：《分子植物育种》2020年第11期3643-3648,共6页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2018YFD0200506);湖北省技术创新专项(2018ABA085)共同资助。

摘　　要：通过对华山新麦草与‘7182’杂交获得的抗条锈病新种质‘H1684’进行遗传分析,明确‘H1684’含有的抗病基因遗传特点。本研究利用基因组原位杂交技术对‘H1684’含有的外源染色体片段进行鉴定,并以‘H1684’、感病对照‘铭贤169’及其杂交后代F1、F2、F2:3和BC1群体为材料,采用10个条锈菌生理小种CYR-23、CYR25、CYR27、CYR28、CYR29、CYR30、CYR31、CYR32、CYR33和CYR34对供试群体进行苗期抗条锈性鉴定,分析抗病基因的遗传规律。GISH分析表明‘H1684’含有来自于华山新麦草的长染色体片段,为华山新麦草易位系。抗病性鉴定表明:‘H1684’在苗期对10个条锈菌生理小种均表现免疫或高抗。‘H1684’对CYR33和CYR34的抗病性都是由1对显性基因控制。华山新麦草易位系‘H1684’对中国目前流行的小麦条锈菌生理小种具有良好的抗病性,可以作为抗源在中国小麦抗条锈病育种中应用。Genetic analysis was performed to determine the inheritance resistance of‘H1684’,a,wheat-Psathyrostachys huashanica germplasm previously developed from the interspecific hybridization between wheat‘7182’and Psathyrostachys huashanica,to stripe rust.Genomic in situ hybridization(GISH)were applied to identify P.huashanica chromosomes in‘H1684’.Furthermore.to confirm the genetic characteristics of resistance gene,ten races of Puccinia striiformis f.sp.tritici(Pst),including CYR23,CYR25,CYR27,CYR28,CYR29,CYR30,CYR31,CYR-32,CYR33 and CYR34,were selected to test the F1,F2,F2:3,BC1 progenies derived from‘H1684’/‘Mingxian 169’in seedling stage in greenhouse.GISH analysis showed that the translocation alien chromatin in H1684 was very long and cytologically detectable,indicating that‘H1684’is a P.huashanica-translocation line.‘H1684’is resistant to the ten tested stripe rust races in seedling stage.Genetic analysis revealed that the resistance of‘H1684’to CYR33 and CYR34 was controlled by a single dominant gene.This study indicated that‘H1684’had good resistance against popular rusts in China and it could be used as a novel resistance resource in breeding rust-resistance wh eat varieties.

关 键 词：华山新麦草(Psathyrostachys huashanica) 小麦条锈病 抗病基因 原位杂交 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]