检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:魏静 杨倩 郑茜 冯跃平 张春 WEI Jing;YANG Qian;ZHENG Xi;FENG Yue-ping;ZHANG Chun(School of Pharmacy,Southwest Medical University,Luzhou,Sichuan,646000,China;Ya'an Fourth People's Hosptial,Yaan,Sichuan,625000,China;Sichuan Vacational College of Health and Rehabilitation,Zigong,Sichuan,643000,China;Hospital(T.C.M)Affiliated to Southwest Medical University,Luzhou,Sichuan,646000,China)
机构地区:[1]西南医科大学药学院,四川泸州646000 [2]雅安市第四人民医院,四川雅安625000 [3]四川卫生康复职业学院,四川自贡643000 [4]西南医科大学附属中医医院,四川泸州646000
出 处:《时珍国医国药》2020年第2期266-270,共5页Lishizhen Medicine and Materia Medica Research
基 金:国家自然科学基金(81001700);四川省科技厅应用基础计划(2012JY0081);四川省科技厅科技项目(14JC0137);西南医科大学青年基金(2018-ZRQN-020)。
摘 要:目的优化筛选大孔树脂富集纯化蝴蝶花中总黄酮的工艺条件,并测定其总黄酮抗肿瘤及抗氧化的生物活性。方法采用静态解析/吸附试验对5种不同型号的大孔树脂进行筛选。以吸附、解析效果为指标,考察大孔树脂纯化黄酮的工艺参数,并利用CCK-8法考察蝴蝶花总黄酮对Hela、Hccc-9810及H460肿瘤细胞株的体外增殖抑制活性;采用铁氰化钾还原法、DPPH法测定蝴蝶花总黄酮体外抗氧化活性。结果 AB-8型大孔树脂用于富集纯化蝴蝶花黄酮效果最好。最佳工艺条件如下:上样液质量浓度为5.00 mg·mL-1,pH值为6.0,吸附4h,上样量5BV,13BV 95%乙醇洗脱。蝴蝶花总黄酮对Hccc-9810及H460肿瘤细胞株具有明显抑制作用(P<0.05),对DPPH自由基有较好的清除能力。蝴蝶花叶总黄酮抗肿瘤及抗氧化活性明显高于其根茎总黄酮。结论 AB-8型大孔树脂适用于蝴蝶花黄酮的分离纯化,确定的工艺条件稳定可行,重复性好。纯化总黄酮具有良好的抗肿瘤及抗氧化活性。Objective To optimize purification technology of flavonoids from Iris japonica Thunb.by macroporous resin,and to e-valuate the antitumor and antioxidant activities of total flavonoids.Methods Five kinds of macroporous resins were screened by the static adsorption and desorption experiments.Adsorption and desorption rate were used as the indices to investigate the tech-nology parameters of the purification of total flavonoids.The antitumor activities of total flavonoids from Iris japonica Thunb.were assayed in Hela/Hccc-9810 and H460 cells by CCK-8 assay.Potassium ferricyanide reduction and DPPH methods were used to evaluate the antioxidant activity of total flavonoids in vitro.Results AB-8 type resin showed the best purifying profile.Its op-timum technology conditions were as follows:sample were adsorpted for 4 h with concentration of 5.00 mg.ml-1 and pH value of 6.0;sample volume was 5BV eluted by 95%ethanol of 13 BV.The total flavonoids showed significant inhibitory activity on Hccc-9810,H460 cells and showed a certain DPPH free radical-scavenging activity.The total flavonoids in the leaves had a stron-ger reducing ability than that in the roots.Conclusion AB-8 is suitable to purify total flavonoids from Iris japonica Thunb.This optimized technology was stable,feasible and good reproducibility.The purified total flavonoids of Iris japonica Thunb.has a cer-tain antioxidant and anti-tumor activities in vitro.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.38