杂色云芝粗多糖预防性给药对扑热息痛诱导的肝损伤小鼠的肝保护作用  

Hepatoprotective Effect of Polysaccharides from Coriolus versicolor on Liver Injury Induced by APAP in Mice

作　　者：白玉莹 葛唯佳 蔡恩博[1] 祝洪艳[1] 包海鹰[1,2] 赵岩 BAI Yuying;GE Weijia;CAI Enbo;ZHU Hongyan;BAO Haiying;ZHAO Yan(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;Engineering Research Center of Ministry of Education for Edible and Medicinal Fungi,Changchun 130118,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]食药用菌教育部工程研究中心,长春130118 [3]北京中医药大学中药学院,北京102488

出　　处：《吉林农业大学学报》2020年第1期56-61,共6页Journal of Jilin Agricultural University

基　　金：教育部长江学者与创新团队项目(IRT_15R25)。

摘　　要：研究杂色云芝粗多糖(YZ0)对对乙酰氨基酚(APAP)诱导的小鼠急性肝损伤的保护作用及其作用机制,结果表明:与模型组相比,YZ0能显著降低小鼠血清ALT、AST、IL-1β、TNF-α、IL-6水平及肝MDA水平,显著增强肝组织中抗氧化酶(SOD、CAT)活性和GSH水平,并显现剂量依赖性。组织病理学观察显示,YZ0对肝损伤有保护作用。此外,YZ0显著抑制了APAP诱导的ERK1/2和JNK的磷酸化,从而减少了促炎细胞因子的分泌和肝细胞凋亡,揭示了YZ0对APAP诱导的小鼠肝毒性具有保护作用,其可能作用机制是减少氧化应激和炎症反应。The present study was designed to investigate the hepatoprotective effect of polysaccharides from Coriolus versicolor(YZ0)and their possible mechanisms in acetaminophen(APAP)-induced acute liver damage in mice.The results indicated that YZ0 significantly decreased serum ALT and AST activities,IL-1β,TNF-αand IL-6 serum levels,hepatic MDA level,and markedly enhanced antioxidant enzyme(SOD and CAT)activity and GSH level in hepatic tissue,in a dose dependent manner,when compared to the APAP group.Histopathological observation revealed the hepatoprotective effect of YZ0 against damage.Furthermore,YZ0 remarkably improved the APAP-induced phosphorylation of ERK1/2 and JNK,therefore to prevent the secretion of pro-inflammatory cytokines and hepatocytes apoptosis.These findings suggested that YZ0 possessed hepatoprotective effect against APAP-induced hepatotoxicity in mice and the action might in part reduce oxidative stress and inflammation.

关 键 词：杂色云芝 多糖 对乙酰氨基酚 药物性肝损伤 

分 类 号：R285.5[医药卫生—中药学] S567.3[医药卫生—中医学]