c-Myc基因在人滋养细胞合体化中的作用  

The Role of c-Myc in the Syncytialization of Human Trophoblast

作　　者：徐增伟[1] 王永恒 刘太行 王应雄[1] 丁裕斌[1] XU Zengwei;WANG Yongheng;LIU Taihang;WANG Yingxiong;DING Yubin(Joint International Research Laboratory of Reproductive and Development,Department of Reproductive Biology,School of Public Health and Management,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]重庆医科大学公共卫生与管理学院,生殖生物学实验室,教育部生殖与发育国际合作联合实验室,重庆400016

出　　处：《中国细胞生物学学报》2020年第3期418-426,共9页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81671493、81801458)资助的课题。

摘　　要：c-Myc属于bHLH家族转录因子,在人多能干细胞维持细胞自我更新中发挥着重要作用,但在人胎盘滋养细胞分化中的功能尚不明确。该研究采用免疫组化、免疫荧光、Western blot及qRT-PCR技术,初步证实人早孕绒毛、原代滋养细胞及福斯科林诱导BeWo细胞合体化前后c-Myc均高表达于细胞滋养细胞,并在滋养细胞合体化过程中表达下调。qRT-PCR检测c-Myc网络相关因子在滋养细胞合体化过程中的作用,发现细胞周期相关因子CCNA2及CDK2表达下调,CDKN2A表达上调;c-Myc调控网络中滋养细胞干性相关因子OCT4、SOX2及hTERT在滋养细胞合体化过程中表达下调。研究初步发现,c-Myc可能通过其调控网络及细胞周期,影响滋养细胞增殖及分化平衡,从而参与滋养细胞合体化。The proto-oncogene c-Myc is known to regulate the self-renewal of human pluripotent stem cells but its function in human trophoblast progenitor cells is unclear.This study aimed to determine the role of c-Myc in the syncytialization of cytotrophoblast cells.In this study,immunohistochemistry,immunofluorescence,Western blot and qRT-PCR were used to detect the expression of c-Myc in human early pregnancy villous trophoblasts and in BeWo cells.Verification of the expression of the transcription factors involved in c-Myc-mediated regulation of syncytialization was done with qRT-PCR.The expression of c-Myc was observed in both the primary villous tissues and Bewo cells.And within the villous tissues,the expression was higher in the inner layer but weaker in the outer layer of the cytotrophoblast.Both forskolin-induced fusion of Bewo cells and spontaneous fusion of the primary cytotrophoblast led to a significant downregulation of c-Myc and the trophoblast stem cell-related transcription factors:OCT4,SOX2 and hTERT.The cell cycle-related factors,CCNA2 and CDK2,were downregulated while CDKN2A was upregulated.The expression of c-Myc was downregulated during syncytialization,and might be involved in the process by regulating the cell cycle and the self-renewal of the trophoblast cells.

关 键 词：滋养细胞 C-MYC BEWO 合体化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]