山楂酸对H2O2诱导的BRL-3A细胞氧化损伤及p38和Nrf2/HO-1通路的影响  

Effects of Maslinic Acid on H2O2-Induced Oxidative Injury and Pathway of p38 and Nrf2/HO-1 in BRL-3A Cells

作　　者：李桂兰[1] 郑开丰 秦韶刚 陈磊[1] 岳彩霞 郭永琪 LI Guilan;ZHENG Kaifeng;QIN Shaogang;CHEN Lei;YUE Caixia;GUO Yongqi(College of Anim al Science and Technology,Shanxi Agricultural University,Jinzhong 030801,China)

机构地区：[1]山西农业大学动物科技学院,晋中030801

出　　处：《中国细胞生物学学报》2020年第3期453-460,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:31301452);山西农业大学引进人才科研启动基金(批准号:XB2012013);山西农业大学科技创新基金(批准号:201313)资助的课题。

摘　　要：该文研究了山楂酸对H2O2诱导的BRL-3A肝细胞氧化损伤的保护作用及其机制。采用MTT法检测细胞活力,DCFH-DA荧光探针法测定细胞的ROS水平,微量酶标法测定细胞LDH、SOD、CAT的活力和GSH、MDA含量;反转录PCR测定细胞Nrf2、HO-1和p38的mRNA表达水平;Western blot检测Nrf2、HO-1和p-p38的蛋白表达水平。结果显示,山楂酸可改善H2O2导致的BRL-3A细胞活力下降,降低ROS水平和LDH渗漏,提高SOD、CAT的活性和GSH含量,降低MDA的水平。此外,山楂酸可促进Nrf2的核转位,提高HO-1的mRNA和蛋白表达水平,抑制p38的磷酸化水平。这些结果表明,山楂酸对H2O2诱导的BRL-3A细胞的氧化损伤有保护作用,其机制与促进Nrf2的核转位、上调HO-1的表达和抑制p38的磷酸化水平可能相关。In the paper,the protective effect and the mechanism of maslinic acid on oxidative injury of BRL-3A cells induced by H2O2 were investigated.Cell viability was determined by MTT.The intracellular ROS levels were measured using DCFH-DA.The activities of LDH,SOD and CAT as well as the contents of GSH and MDA were assayed by micro-enzyme-linked method.The mRNA levels of Nrf2,HO-1 and p38 were investigated by reverse transcription polymerase chain reaction.Western blot was used to evaluate the protein levels of Nrf2,HO-1 and p-p38.The results showed that maslinic acid attenuated the cell viability loss,the intracellular ROS levels and LDH leakage induced by H2O2.The activities of SOD and CAT,the GSH content were increased,but the level of MDA was reduced by maslinic acid.In addition,the nuclear translocation of Nrf2 could be promoted.The expression of HO-1 both in mRNA level and protein level was up-regulated.The phosphorylation of p38 was inhibited by maslinic acid.These results suggested that maslinic acid protected against H2O2-induced oxidative injury in BRL-3A cells,and its mechanism was probably associated with the promotion of Nrf2 nuclear translocation,the up-regulation of HO-1 expression and the inhibition of p38 phosphorylation.

关 键 词：山楂酸 BRL-3A细胞 P38 核因子E2相关因子2 血红素氧化酶-1 

分 类 号：R285.5[医药卫生—中药学]