Extensive Inter-plant Protein Transfer between Cuscuta Parasites and Their Host Plants  

作　　者：Nian Liu Guojing Shen Yuxing Xu Hui Liu Jingxiong Zhang Shalan Li Jing Li Cuiping Zhang Jinfeng Qi Lei Wang Jianqiang Wu 

机构地区：[1]Department of Economic Plants and Biotechnology,Yunnan Key Laboratory for Wild Plant Resources,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China [2]University of the Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Molecular Plant》2020年第4期573-585,共13页分子植物（英文版）

基　　金：This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB11050200);the National Science Foundation of China(31970274);CAS Youth Innovation Prom otion Association(2018426);the International Partnership Program of the Chinese Academy of Sciences(151853KYSB20170025).

摘　　要：Cuscuta species(dodders)are holoparasites that totally rely on host plants to survive.Although various mobile proteins have been identified to travel within a plant,whether and to what extent protein transfer between Cuscuta and host plants remain unclear.We found that hundreds to more than 1500 proteins were transferred between Cuscuta and the host plants Arabidopsis and soybean,and hundreds of interplant mobile proteins were even detected in the seeds of Cuscuta and the host soybean.Different hosts bridge-connected by dodder were also found to exchange hundreds of proteins.Quantitatively,the mobile proteins represent a few to more than 10%of the proteomes of foreign plants.Using Arabidopsis plants expressing different reporter proteins,we further showed that these reporter proteins could travel between plants and,importantly,retained their activity in the foreign plants.Comparative analysis between the interplant mobile proteins and mRNAs indicated that the majority of mobile proteins were not de novo synthesized from the translocated mRNAs,but bona fide mobile proteins.We propose that large-scale inter-plant protein translocation may play an important role in the interactions between host plants and dodder and even among the dodder bridge-connected hosts.

关 键 词：DODDER CUSCUTA host plants protein transfer plant-plant interaction 

分 类 号：Q94[生物学—植物学]