Histone Deacetylase HDA9 and WRKY53 Transcription Factor Are Mutual Antagonists in Regulation of Plant Stress Response  

作　　者：Yu Zheng Jingyu Ge Chun Bao Wenwen Chang Jingjing Liu Jingjie Shao Xiaoyun Liu Lufang Su Lei Pan Dao-Xiu Zhou 

机构地区：[1]Institute for Interdisciplinary Research and Hubei Province Engineering Research Center of Legume Plants,Jianghan University,Wuhan 430056,China [2]Institute of Plant Sciences Paris-Saclay,CNRS,INRAE,Universite Paris-Saclay,Orsay 91405,France

出　　处：《Molecular Plant》2020年第4期598-611,共14页分子植物（英文版）

基　　金：This study was supported by the Discipline Innovation Team Foundation of Jianghan University(03100074);Natural Science Foundation of Hubei Province(grant no.2016CFB630);National Natural Science Foundation of China(NSFC31701100,NSFC31600981,and NSFC31600801);French ANR-19-CE12-0027-01.

摘　　要：Epigenetic regulation of gene expression is important for plant adaptation to environm ental changes.Previous results showed that Arabidopsis RPD3-like histone deacetylase HDA9 is known to function in repressing plant response to stress in Arabidopsis.However,how HDA9 targets to specific chromatin loci and controls gene expression netw orks involved in plant response to stress remains largely unclear.Here,we show that HDA9 represses stress tolerance response by interacting with and regulating the DNA binding and transcriptional activity of WRKY53,which functions as a high-hierarchy positive regulator of stress response.We found that WRKY53 is post-translationally modified by lysine acetylation at multiple sites,some of which are removed by HDA9,resulting in inhibition of WRKY53 transcription activity.Conversely,WRKY53 negatively regulates HDA9 histone deacetylase activity.Collectively,our results indicate that HDA9 and WRK53 are reciprocal negative regulators of each other's activities,illustrating how the functional interplay between a chromatin regulator and a transcription factor regulates stress tolerance in plants.

关 键 词：HDA9 WRKY53 stress lysine acetylation transcription activity 

分 类 号：Q943.2[生物学—植物学]