Identification of Naturally Occurring Polyamines as Root-Knot Nematode Attractants  

作　　者：Morihiro Oota Allen Yi-Lun Tsai Dan Aoki Yasuyuki Matsushita Syuuto Toyoda Kazuhiko Fukushima Kentaro Saeki Kei Toda Laetitia Perfus-Barbeoch Bruno Favery Hayato Ishikawa Shinichiro Sawa 

机构地区：[1]Graduate School of Science and Technology,Kumamoto University,Kurokami 2-39-1,Kumamoto 860-8555,Japan [2]Graduate School of Bioagricultural Sciences,Nagoya University,Nagoya 464-8601,Japan [3]INRA,Universite Cote d'Azur,CNRS,UMR 1355-7254 Institut Sophia Agrobiotech,Sophia-Antipolis 06900,France [4]Present address:Dormancy and Adaptation Research Unit,RIKEN Center for Sustainable Resource Science,Yokohama 230-0045,Japan

出　　处：《Molecular Plant》2020年第4期658-665,共8页分子植物（英文版）

基　　金：This work was supported by JSPS KAKENHI grants(25252032 and 15H01230 to K.F.,16H04168 to K.T.,24114009,24370024,16K14757,17H03967,and 18H05487 to S.S.);L.P.-B.and B.F.are supported by INRA and the French Government(National Research Agency,ANR)through the"Investments for the Future"LABEX SIGNALIFE program,reference no.ANR-11-LABX-0028-01.

摘　　要：Root-knot nematodes(RKNs;genus Meloidogyne)are a class of plant parasites that infect the roots of many plant species.It is believed that RKNs target certain signaling molecules derived from plants to locate their hosts;however,currently,no plant compound has been unambiguously identified as a universal RKN attractant.To address this question,we screened a chemical library of synthetic compounds for Meloidogyne incognita attractants.The breakdown product of aminopropylam ino-anthraquinone,1,3-diam inopropane,as well as its related compounds,putrescine and cadaverine,were found to attract M.incognita.After exam ining various polyamines,M.incognita were found to be attracted specifically by natural com pounds that possess three to five methylene groups between two terminal amino groups.Using cryo-TO F-SIM S/SEM,cadaverine was indeed detected in soybean root cortex cells and the surrounding rhizosphere,establishing a chemical gradient.In addition to cadaverine,putrescine and 1,3-diam inopropane were also detected in root exudate by HPLC-MS/MS.Furtherm ore,exogenously applied cadaverine is sufficient to enhance M.incognita infection of Arabidopsis seedlings.These results suggest that M.incognita is likely attracted by polyam ines to locate the appropriate host plants,and the naturally occurring polyamines have potential applications in agriculture in developing protection strategies for crops from RKN infection.

关 键 词：plant-parasitic nematode CHEMOTAXIS RHIZOSPHERE organic diamine 

分 类 号：Q94[生物学—植物学]