黑脉羊肚菌固体培养基的制备及其培养条件优化  

Preparation and Optimization of Solid Media of Morchella angusticeps

作　　者：张保顺[1] 叶孟婷 刘清 王建康 罗楠轩 杨昌庆 朱昱蓉 李逐波[1] 刘元喜 ZHANG Bao-shun;YE Meng-ting;LIU Qing;WANG Jian-kang;LUO Nan-xuan;YANG Chang-qing;ZHU Yu-rong;LI Zhu-bo;LIU Yuan-xi(School of Pharmaceutical Sciences and Chinese Medicine, Southwest University, Chongqing 400715, China;Qianjiang Agricultural and Rural Committee, Qianjiang Chongqing 409000, China)

机构地区：[1]西南大学药学院·中医药学院,重庆400715 [2]重庆市黔江区农业农村委员会,重庆黔江409000

出　　处：《西南师范大学学报（自然科学版）》2020年第6期52-62,共11页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：重庆市科学技术局社会事业与民生保障科技创新专项(cstc2017shms-zdyfX0061,cstc2017shms-xdny80016)。

摘　　要：以黑脉羊肚菌为对象,研究了不同固体培养基对菌种生长的影响.通过以菌丝生长速度、密度和菌核为考察指标,研究在不同碳源、氮源、无机盐及pH条件下羊肚菌菌丝体的生长状况,从而优化培养基的制备工艺.结果表明:黑脉羊肚菌生长的最适条件为2%的葡萄糖碳源,2 g/L的蛋白胨氮源,MgSO4无机盐,pH值为6.0.该实验为今后羊肚菌的栽培奠定了坚实的基础.Morchella anguisticeps is a wild edible fungus.In order to lay a basis for its cultivation,an experiment was made to study the effects of different solid culture media on the growth of the fungus strain.With mycelial growth rate and density and sclerotia formation as the major.indicators,the growth of its mycelia in culture media of different carbon and nitrogen sources,inorganic salts and pH was investigated so as to optimize the preparation process of culture media.The resulted revealed that the optimum formula of the culture media for the growth of M.angusticeps was 2%glucose as the carbon source,2 g/L peptone as the nitrogen source,MgSO4 as the inorganic salt and a pH of 6.0.

关 键 词：羊肚菌 菌丝体 培养条件 筛选 

分 类 号：S646.7[农业科学—蔬菜学]