顶空-气相色谱/质谱法检验血液中的氧化亚氮  

Determination of Nitrous Oxide in Blood Using Headspace Gas Chromatography-mass Spectrometry

作　　者：胡浩男 蒋国军[1] 唐志龙 历洪光 梁栋[1] HU Haonan;JIANG Guojun;TANG Zhilong;LI Hongguang;LIANG Dong(Criminal Police Detachment of Xuzhou Public Security Bureau,Xuzhou 221000,Jiangsu,China)

机构地区：[1]徐州市公安局刑警支队,江苏徐州221000

出　　处：《刑事技术》2020年第3期250-254,共5页Forensic Science and Technology

摘　　要：目的建立氧化亚氮中毒案件中血液检材氧化亚氮的快速、准确的定性定量检验方法。方法血样1mL置于20mL顶空瓶中,加入内标二氯甲烷1μL密封,置室温下平衡10min后,放入顶空器70℃加热10min后抽取上层气体300μL进样气相色谱-质谱联用仪(GC-MS)。结果血液中氧化亚氮在0.02~5.0mL/mL浓度范围内线性关系良好,检出限为0.005mL/mL血,并在两起氧化亚氮中毒案件中成功应用。结论本文建立的方法快捷、科学、有效,可应用于血液检材中氧化亚氮的定性定量检验。Objective To establish a fast and accurate method for determination of nitrous oxide in blood from the related poisoning cases.Methods 1 mL blood sample was collected into a 20mL headspace bottle that was afterwards added of 1μL dichloromethane,the internal standard,having successively been sealed and left for 10 min at room temperature.The samplecontained headspace bottle was heated under 70℃for another 10 min to make the relevant substance gasify,with the 300μL bottled upper gas being injected into the GC-MS for analysis.Results A good linearity was observed at the mass concentration of nitrous oxide within 0.02-5.0mL/mL in blood,with the limit of detection(LOD)being 0.005mL/mL.Applied with the established method,nitrous oxide was detected successfully from the victims’blood samples of two cases of sulfi de poisoning.Conclusion The method is fast,scientifi c and effective,applicable for rapid detection of nitrous oxide from the involving poisoning cases.

关 键 词：法医毒物学 氧化亚氮 中毒案件 顶空-气相色谱/质谱 

分 类 号：DF795.1[医药卫生—法医学]