碳量子点荧光猝灭法检测药物中的异鼠李素  

Facile Synthesis of Fluorescence Carbon Quantum Dots and Their Application in Detection of Isorhamnetin in Medicines

作　　者：李海红 刘荔贞[2] 冯锋[1,2] LI Hai-hong;LIU Li-zhen;FENG Feng(School of Chemistry and Materials Science,Shanxi Normal University,Linfen 041004,China;College ofChemistry and Chemical Engineering,Shanxi Datong University,Datong 037009,China)

机构地区：[1]山西师范大学化学与材料科学学院,山西临汾041004 [2]山西大同大学化学与环境工程学院,山西大同037009

出　　处：《分析测试学报》2020年第4期514-519,共6页Journal of Instrumental Analysis

基　　金：山西省教育厅高等学校科技创新项目(2019L0750);大同市重点研发计划项目基金(2018014)。

摘　　要：以L-天冬氨酸为碳源,尿素为氮源,采用微波加热法制备荧光碳量子点(CDs),得到的CDs具有球形结构和单一分散性,平均粒径约为5 nm。红外光谱、X射线光电子能谱、紫外和荧光光谱的表征结果表明,合成的CDs具有较高的荧光稳定性,良好的水溶性和对异鼠李素(ISOR)高的选择性。在优化条件下,ISOR浓度在0.22~180 nmol/L范围内时与CDs荧光猝灭程度(IF0/IF)呈良好的线性关系,检出限(LOD)为1.32 nmol/L,回收率为90.8%~107%。结果表明,该CDs可用于ISOR的快速、高效、灵敏检测。A kind of novel fluorescence carbon quantum dots(CDs)were fabricated by microwave heating method using L-aspartic acid and urea as the precursors.The obtained CDs exhibited good spherical shapes with an average size about 5 nm.In addition,the CDs were characterized by Fourier transform infared spectroscopy,X-ray photoelectron spectroscopy,ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy.The CDs showed high fluorescence stability,good particle dispersion,excellent water solubility and remarkable selectivity toward isorhamnetin(ISOR).Under the optimized conditions,there was a good relationship between fluorescence quenched degree(IF0/IF)of the CDs and concentration of ISOR in the range of 0.22-180 nmol/L with a limit of detection(LOD)of 1.32 nmol/L.The CDs were successfully applied in the detection of ISOR in ginkgo biloba tablets with recoveries of 90.8%-107%.Results showed that the as-synthesized CDs could be used in ISOR detection with rapidness,efficiency and sensitivity.

关 键 词：L-天冬氨酸 尿素 碳量子点 异鼠李素 猝灭 

分 类 号：O657.3[理学—分析化学]