基于共价有机骨架修饰电极的苯并芘电化学发光传感器  

A Benzopyrene Electrochemical Luminescence Sensor Based on Covalent Organic Framework Modified Electrode

作　　者：孙苗 徐文清 王黎 庞月红[1] SUN Miao;XU Wen-qing;WANG Li;PANG Yue-hong(School of Food Science and Technology,Jiangnan University,Wuxi214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《分析测试学报》2020年第5期577-582,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21976070);中央高校基本科研业务费专项资金(JUSRP22003)。

摘　　要：苯并芘(BaP)是一种常见的多环芳烃类化合物,由于其对人体具有强烈的致癌性而引起广泛关注。该文将1,3,5-三醛基间苯三酚(CTp)与2,5-二甲基对苯二胺(Pa-2)经缩合反应形成的共价有机骨架材料CTpPa-2通过滴涂法修饰于电极表面,并与鲁米诺-过氧化氢体系相结合,构建了一种简单快速、稳定性好的BaP电化学发光传感器。通过扫描电子显微镜、差分脉冲伏安法、循环伏安法、恒电流充放电法及电化学发光法表征CTpPa-2修饰电极的形貌和电化学性能,并考察对BaP测定的可行性。在优化实验条件下,发光强度与苯并芘浓度在50~600 nmol/L范围内呈良好的线性关系,相关系数(r^2)为0.9951,检出限(S/N=3)为5.60 nmol/L。将该方法应用于烤肉样品中BaP的检测,结果与高效液相色谱(HPLC)法相近,表明该方法具有良好的应用前景。Benzopyrene(BaP)is a common polycyclic aromatic hydrocarbon compound,which has attracted wide attention due to its strong carcinogenicity to the human body.In this paper,an electrochemical luminescence sensor for the detection of BaP was constructed based on a covalent organic framework modified electrode.Firstly,the covalent organic framework material,CTpPa-2 formed by the condensation reaction of 1,3,5-triformylphloroglucinol(CTp)and 2,5-dimethyl-p-phenylenediamine(Pa-2),was modified on the electrode surface by drop coating method,then a simple and rapid BaP electrochemical luminescence sensor with good stability was constructed by combining the modified electrode with a luminol-hydrogen peroxide system.The morphology and electrochemical properties of CTpPa-2 modified carbon electrode were characterized by scanning electron microscopy,differential pulse voltammetry,cyclic voltammetry,galvanostatic charge/discharge and electrochemical luminescence.The feasibility for the determination of BaP was also investigated.Under the optimized experimental conditions,there was a good linear relationship between luminescent intensity and BaP concentration in the range of 50-600 nmol/L with a correlation coefficient(r^2)of 0.9951,and the limit of detection(S/N=3)was 5.60 nmol/L.The proposed sensor was applied in the detection of BaP in roasted meat sample,and the results obtained were similar to those of HPLC method,indicating that this method has a good application prospect.

关 键 词：共价有机骨架 苯并芘(BaP) 鲁米诺 电化学发光 传感器 

分 类 号：O657.1[理学—分析化学] O625.14[理学—化学]