火山岩填料曝气生物滤池的SNAD工艺启动特性及功能菌丰度演替  

Start-up Characteristics of SNAD Process and Functional Abundance Succession of Volcanic Rock Biological Aerated Filter

作　　者：薛嘉俊 张绍青[1,3] 张立秋 李淑更[2,3] 姚海楠 耿忠轩[1,2] 李鸿 刘晓玲 XUE Jia-jun;ZHANG Shao-qing;ZHANG Li-qiu;LI Shu-geng;YAO Hai-nan;GENG Zhong-xuan;LI Hong;LIU Xiao-ling(School of Civil Engineering,Guangzhou University,Guangzhou 510006,China;Key Laboratory for Water Quality and Conservation of the Pearl River Delta,Ministry of Education,Guangzhou University,Guangzhou 510006,China;School of Environmental Science and Engineering,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]广州大学土木工程学院,广州510006 [2]广州大学珠江三角洲水质安全与保护教育部重点实验室,广州510006 [3]广州大学环境科学与工程学院,广州510006

出　　处：《环境科学》2020年第6期2796-2804,共9页Environmental Science

基　　金：国家自然科学基金项目(51478127,51708140);广州市科技计划项目(201510010051)。

摘　　要：为研究同步亚硝化、厌氧氨氧化耦合反硝化(SNAD)工艺在浸没式生物滤池反应器(SBAF)内的运行特性,同时接种亚硝化污泥和富集ANAMMOX的填料启动SNAD反应器.结果表明在60 mg·L^-1有机物浓度下,自养脱氮和反硝化实现较好的耦合,并在该浓度下稳定运行了67 d,其总氮去除率最高可达92.0%,COD去除率最高达82.9%,最高总氮去除负荷为2.3 kg·(m^3·d)^-1.与全程自养脱氮(CANON)工艺相比,SNAD工艺的平均总氮去除率提高了12.6%.荧光定量PCR结果显示,系统启动后AOB菌的丰度有所增长,ANAMMOX菌的丰度增长了1个数量级,而NOB菌和反硝化菌的数量维持在较低水平(小于107 copies·g^-1),表明以火山岩为填料的浸没式生物滤池反应器有利于ANAMMOX和AOB的协同生长,可快速实现SAND工艺的启动.A SNAD(simultaneous partial nitrification,ANAMMOX,and denitrification)process initiated in a submerged biological aerated filter(SBAF)was started up by seeding nitrification sludge and an ANAMMOX filter to investigate the operating characteristics and the succession of functional bacteria.The results showed that when the autotrophic nitrogen removal and denitrification were operated stably for 67 days at an initial COD concentration of 60 mg·L^-1,the maximum nitrogen removal efficiency,the COD removal rate,and the nitrogen removal rate were 92.0%,82.9%,and 2.3 kg·(m^3·d)^-1,respectively.Moreover,the total nitrogen removal rate of the SNAD process in this study was 12.6%higher than that of CANON process.The results of quantitative PCR showed that the abundance of AOB slightly increased,while the abundance of ANAMMOX was one order higher than that before the start-up of SNAD.In comparison,the abundances of denitrifiers and NOB remained at a relatively low level(107 copies·g^-1).Taken together,these results suggested that the SBAF process with volcanic filter was beneficial for the accumulation of ANAMMOX and AOB.

关 键 词：SNAD工艺 CANON工艺 火山岩填料 生物滤池 定量PCR 

分 类 号：X703[环境科学与工程—环境工程]