游离羟胺对两种典型亚硝态氮氧化菌活性的影响  

Effect of Free Hydroxylamine on the Activity of Two Typical Nitrite-oxidizing Bacteria

作　　者：沈琛 张树军[2] 彭永臻[1] SHEN Chen;ZHANG Shu-jun;PENG Yong-zhen(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Beijing University of Technology,Beijing 100124,China;Research and Development Center of Beijing Drainage Group Corporation,Beijing 100022,China)

机构地区：[1]北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室,北京100124 [2]北京城市排水集团有限责任公司科技研发中心,北京100022

出　　处：《环境科学》2020年第6期2805-2811,共7页Environmental Science

基　　金：国家重点研发计划项目(2016YFC0401103);北京市科技计划项目(D171100001017001,Z181100005518003);北京市教委资助项目。

摘　　要：为考察羟胺对两种典型亚硝态氮氧化菌(NOB)活性的影响,分别以富含硝化杆菌(Nitrobacter)和富含硝化螺菌(Nitrospira)的污泥作为研究对象,基于批次试验,分别考察了相同pH、不同羟胺浓度梯度和相同羟胺浓度、不同pH梯度条件下,羟胺对两种NOB的抑制效果.结果表明在相同pH条件下,Nitrobacter的活性随羟胺浓度的升高而降低;在相同羟胺浓度(HA=5mg·L^-1)条件下,羟胺在较高pH环境(pH≥7.5)中产生更多的游离羟胺(FHA),会对Nitrobacter产生更好的抑制效果,低pH环境(pH≤7)中离子态羟胺的存在可能会促进Nitrobacter的活性.羟胺对Nitrospira的抑制效果有限,当pH=7.5,羟胺浓度=45mg·L^-1时,Nitrospira的相对活性为82%.分别采用NOB生长速率动力学模型和非底物抑制线性方程描述FHA对Nitrobacter和Nitrospira活性的影响,其可决系数R2分别为0.90和0.94,FHA可能是抑制Nitrobacter和Nitrospira活性的主要原因.The sludge from enrichment of Nitrobacter and Nitrospira was used as a research object and batch tests were performed.The inhibitory effects of hydroxylamine on Nitrobacter and Nitrospira under the same pH and different hydroxylamine concentration gradients,the same hydroxylamine concentration,and different pH gradients were investigated.The results showed that under the same pH condition,the activity of Nitrobacter decreased with increasing hydroxylamine concentration.Under the same hydroxylamine concentration(HA=5 mg·L^-1)at a higher pH environment(pH≥7.5),hydroxylamine produced more free hydroxylamine(FHA)and the inhibitory effect on Nitrobacter was improved.At a low pH environment(pH≤7),ionic hydroxylamine promoted the activity of Nitrobacter.The inhibitory effect of hydroxylamine on Nitrospira was limited.When pH=7.5 and hydroxylamine concentration was 45 mg·L^-1,the relative activity of Nitrospira was 82%.The NOB growth rate kinetics model and the non-substrate inhibition linear equation were used to describe the effect of FHA on Nitrobacter and Nitrospira activity.The coefficient of determination R2 was 0.90 and 0.94,respectively.FHA may be the main reason for inhibiting the activity of Nitrobacter and Nitrospira.

关 键 词：硝化杆菌 硝化螺菌 相对活性 羟胺 游离羟胺 

分 类 号：X703[环境科学与工程—环境工程]