不同强度耐力运动干预下生长期大鼠骨细胞代谢水平的变化  

Changes of bone metabolism after different intensity endurance exercises in growing rats

作　　者：陈泽刚[1] 丁海丽[2] 李龙[1] 王纯[2] Chen Zegang;Ding Haili;Li Long;Wang Chun(Graduate School,Chengdu Sport University,Chengdu 610041,Sichuan Province,China;School of Sport and Health,Chengdu Sport University,Chengdu 610041,Sichuan Province,China)

机构地区：[1]成都体育学院研究生院,四川省成都市610041 [2]成都体育学院运动与健康学院,四川省成都市610041

出　　处：《中国组织工程研究》2020年第35期5582-5588,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家社科基金一般项目(19BTY124),课题名称:成人慢性非特异性腰痛运动疗法的科学化及应用策略研究,项目参与人:陈泽刚。

摘　　要：背景:随着社会的发展和人们生活水平的提升,处于生长发育敏感期的青少年健康日益受到关注。运动可改善骨细胞代谢,但有关耐力运动对生长期大鼠骨细胞代谢水平影响方面的研究不多。目的:探讨不同强度耐力运动对生长期大鼠骨细胞代谢水平的影响。方法:实验方案经成都体育学院运动与健康学院动物伦理委员会批准。选择40只大鼠适应性训练1周后,随机分为对照组、低强度耐力运动组、中强度耐力运动组、高强度耐力运动组。对照组大鼠常规饲养,不进行训练干预。各运动实验组训练12周,每周训练5 d,每天训练60 min。每2周进行一次最大摄氧量测试,根据测试结果调整后2周的训练负荷。小、中、大强度耐力运动组主负荷期的运动强度为分别为50%,65%及80%最大摄氧量。运动训练实验后观察各组大鼠骨密度、骨结构、骨代谢的指标变化。结果与结论:①关于大鼠股骨骨密度、全身骨密度,运动实验后,小、中等强度耐力运动组与对照组相比均有改善(P<0.05);大强度耐力运动组优于小强度耐力运动组、对照组(P<0.01)。小、中强度耐力运动组运动实验前、后相比差异有显著性意义(P<0.05);大强度耐力运动组运动实验前、后相比差异有非常显著性意义(P<0.01)。②关于大鼠骨小梁面积,运动实验后,小强度耐力运动组、中强度耐力运动组与对照组相比均有改善(P<0.05);大强度耐力运动组显著优于对照组(P<0.01);大强度耐力运动组优于小、中强度耐力运动组(P<0.05)。③关于大鼠股骨组织中骨钙素、碱性磷酸酶、抗酒石酸酸性磷酸酶等指标,运动实验后,小、中强度耐力运动组与对照组相比均有改善(P<0.05);大强度耐力运动组显著优于对照组(P<0.01);大强度耐力运动组优于小、中强度耐力运动组(P<0.05)。④关于大鼠血清中骨钙素、碱性磷酸酶、抗酒石酸酸性磷酸酶等指标,�BACKGROUND:With the development of society and the improvement of people's living standards,the health of adolescents who are being in a period of sensitive growth and development has attracted increasing attention.Exercise can improve bone metabolism,but less is reported on the effect of endurance exercise on bone metabolism in growing rats.OBJECTIVE:To investigate the effect of different intensity endurance exercises on bone metabolism in growing rats.METHODS:The experimental protocol was approved by the Animal Ethics Committee of the School of Sport and Health of Chengdu Sport University.After 1 week of adaptive training,40 rats were randomly divided into three groups:quiet control group,low intensity endurance group,middle intensity endurance group and high intensity endurance group.The rats in the quiet control group were fed regularly without training intervention.The rats in the other three groups were trained,60 minutes a day,5 days per week,for 12 weeks.The maximum oxygen uptake test was conducted every 2 weeks,based on which the training load in the next 2 weeks was adjusted.The load intensity level in the main load period was defined as the percentage of maximum oxygen uptake(VO2max),which was 50%VO2max in the low intensity group,65%VO2max in the middle intensity group and 80%VO2max in the high intensity group.Changes in bone density,bone structure and bone metabolism in each group were observed after exercise.RESULTS AND CONCLUSION:Compared with the quiet control group,there was a significant improvement in rat femoral density and whole body bone density in all the intensity groups after exercise(P<0.05),and the high intensity group had better outcomes than the low intensity group and quiet control group(P<0.01).There were significant differences in the low and middle intensity groups(P<0.05)and extremely significant difference in the high intensity group(P<0.01)before and after exercise.Compared with the quiet control group,trabecular bone area and levels of osteocalcin,alkaline phosphatase,and tartr

关 键 词：骨密度 骨结构 骨代谢 耐力运动干预 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]