Novel synthesis method combining a foaming agent with freeze-drying to obtain hybrid highly macroporous bone scaffolds  

作　　者：Paulina Kazimierczak Aleksandra Benko Krzysztof Palka Cristina Canal Dorota Kolodynska Agata Przekora 

机构地区：[1]Department of Biochemistry and Biotechnology,Medical University of Lublin,Chodzki 1,20-093,Lublin,Poland [2]AGH University of Science and Technology,Faculty of Materials Science and Ceramics,A.Mickiewicza 30 Ave.,30-059,Krakow,Poland [3]Department of Materials Engineering,Lublin University of Technology,Nadbystrzycka 36,20-618,Lublin,Poland [4]Biomaterials,Biomechanics and Tissue Engineering Group,Department of Materials Science and Metallurgy,Universitat Politècnica de Catalunya,Barcelona,08019,Spain [5]Research Centre in Multiscale Science and Engineering,Universitat Politècnica de Catalunya,Barcelona,08019,Spain [6]Department of Inorganic Chemistry,Maria Curie-Sklodowska University,Maria Curie Sklodowska Sq.2,20-031,Lublin,Poland

出　　处：《Journal of Materials Science & Technology》2020年第8期52-63,共12页材料科学技术（英文版）

基　　金：financially supported by the National Science Centre(NCN)in Poland within OPUS 16(No.UMO2018/31/B/ST8/00945);Analysis(ATR-FTIR)performed by Aleksandra Benko was supported by the National Science Centre(NCN)in Poland(No.UMO-2017/24/C/ST8/00400);financial support through Project PCIN2017-128/AEI;Ramon y Cajal fellowship of CC。

摘　　要：Three-dimensional macroporous scaffolds are commonly used in bone tissue engineering applications since they provide sufficient space for cell migration and proliferation, facilitating bone ingrowth and implant vascularisation. The aim of this work was to combine two simple methods, freeze-drying and gas-foaming, in order to fabricate highly macroporous bone scaffolds made of chitosan/agarose matrix reinforced with nanohydroxyapatite. The secondary goal of this research was to comprehensively assess biomedical potential of developed biomaterials. In this work, it was demonstrated that simultaneous application of freeze-drying and gas-foaming technique allows to obtain hybrid(as proven by ATR-FTIR)macroporous bone scaffolds(pore diameter > 50 um) characterized by high open(70%) and interconnected porosity. Novel scaffolds were non-toxic, favoured osteoblasts adhesion and growth and induced apatite formation on their surfaces, indicating their high bioactivity that is essential for good implant osseointegration. Biomaterials were also prone to enzymatic degradation, degradation in acidified microenvironment(e.g. osteoclast-mediated), and slow degradation under physiological p H of 7.4.Moreover, the scaffolds revealed microstructure(70% open porosity, SSA approx. 30 m2/g, high share of macropores with diameter in the range 100-410 um) and compressive strength(1–1.4 MPa) comparable to cancellous bone, indicating that they are promising implants for cancellous bone regeneration.

关 键 词：AGAROSE Chitosan Nanohydroxyapatite Biomaterial porosity BIODEGRADATION CRYOGEL 

分 类 号：R318.08[医药卫生—生物医学工程]