SERS-encoded nanocomposites for dual pathogen bioassay  

作　　者：Yaqi Shan Mingliang Wang Zengliang Shi Milan Lei Xiaoxuan Wang Fu-Gen Wu Huan-Huan Ran Gowri Manohari Arumugam Qiannan Cui Chunxiang Xu 

机构地区：[1]State Key Laboratory of Bioelectronics,School of Chemistry and Chemical Engineering,Southeast University,Nanjing 211189,China

出　　处：《Journal of Materials Science & Technology》2020年第8期161-167,共7页材料科学技术（英文版）

基　　金：supported financially by the National Key Research and Development Program of China(Nos.2018YFA0209101 and 2017YFA0700503);the National Natural Science Foundation of China(Nos.11734005 and 61704024);the Science&Technology Project of Jiangsu Province(Nos.BE2016177 and BK20170696);the Fundamental Research Funds for the Central Universities;Zhishan Young Scholar Fund;the Priority Academic Program Development of Jiangsu Higher Education Institutions。

摘　　要：Surface-enhanced Raman spectroscopy(SERS)has been successfully applied to detect various biomolecules,but it is still in challenge to assay living cells or bacteria sensitively,selectively and quantitatively in complex environments.In this paper,4-ATP and DTNB are assembled on Ag nanoparticle(NP)-decorated poly(styrene-co-acrylic acid)(PSA)nanospheres and then sealed by silica shells to form sensitive SERS labels based on the localized surface plasmon resonance of Ag NPs and large light scattering cross-sections of PSA nanospheres.They are further developed as encoding tags for dual detection of S.aureus and E.coli after assembling corresponding aptamers,which demonstrate ultralow detection limits of 8 cell L-1 for S.aureus and 2 cell L-1 for E.coli.Such a bioassay indicates a point-of-care strategy of ultrasensitively biomedical detections by encoding specific SERS tags.

关 键 词：SERS PATHOGENS Dual detection Encoded nanocomposites Complex environments 

分 类 号：O657.37[理学—分析化学] R318.08[理学—化学]