脱氢弯孢霉菌素对脂多糖诱导的巨噬细胞炎症研究  

LPS-simulated inflammatory response by dehydrocurvularin in macrophages

作　　者：钟静 赵群 李玉兰 余贤军 邓张双 ZHONG Jing;Zhao qun;Li Yulan;Yu Xianjun;DENG Zhangshuang(College of Biological and Pharmaceutical Sciences,China Three Gorges University,Yichang 443002,Hubei,China;Department of biochemistry,Faculty of Basic Medicine,Hubei University of Medicine,Shiyan 442000,Hubei,China;Yichang Central People’s Hospital,the First Clinical Medical College of China Three Gorges University,Yichang 443003,Hubei,China)

机构地区：[1]三峡大学生物与制药学院,湖北宜昌443002 [2]湖北医药学院基础医学院生物化学教研室,湖北十堰442000 [3]宜昌市中心人民医院,三峡大学第一临床医学院,湖北宜昌443003

出　　处：《生物资源》2020年第2期248-253,共6页Biotic Resources

基　　金：湖北省教育厅科研计划项目(D20182101)。

摘　　要：研究脱氢弯孢酶菌素(Dehydrocurvularin,DCV)对脂多糖(lipopolysaccharide,LPS)诱导的小鼠腹腔(peritoneal macrophages,PMs)和骨髓来源的巨噬细胞(bone-marrow-derived macrophages,BMDMs)的影响。MTT方法筛选最佳药物浓度处理不同来源的巨噬细胞,采用Western blot方法检测诱导型一氧化氮合酶(induceble nitric oxide synthase,iNOS)、环氧化酶-2(cyclooxygenase-2,COX-2)及炎性信号通路NF-κB和MAPK的相关蛋白的表达。结果显示,脱氢弯孢酶菌素可显著抑制LPS诱导的iNOS、COX-2的表达,且呈剂量依赖性。NF-κB和MAPK信号通路相关的蛋白激活也受到了明显的抑制。结果表明,脱氢弯孢酶菌素可显著抑制LPS诱导的不同来源的免疫细胞的炎症。To study the effect of dehydrocurvularin(DCV)in lipopolysaccharide(LPS)-induced mouse peritoneal macrophages(PMs)and bone-derived macrophages(bone-marrow-derived macrophages,BMDMs). MTT was used to screen the optimal drug concentration to treat macrophages from different sources. Western-blot method was used to detect cyclooxygenase-2(COX-2)and inflammatory signaling pathway NF-κB and MAPK related proteins. DCV significantly inhibited LPS-induced COX-2 expression in a dose-dependent manner. Protein activation associated with NF-κB and MAPK signaling pathways was also significantly inhibited. DCV inhibits LPS-induced inflammatory properties of immune cells from different sources.

关 键 词：脱氢弯孢霉菌素 炎症 NF⁃κB通路 MAPK通路 

分 类 号：R285.5[医药卫生—中药学]