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作 者:姜莲[1] 王影超 廖敏[1] 许亚玲[1] JIANG Lian;WANG Yingchao;LIAO Min;XU Yaling(Guizhou Institute for Food and Drug Control,Guiyang 550004,China)
出 处:《中国民族民间医药》2020年第11期23-26,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy
基 金:国家药典委员会国家药品标准提高行动计划项目(NO.89)。
摘 要:目的:研究并控制康妇炎胶囊中苦参的质量。方法:采用薄层色谱法对苦参进行鉴别研究;采用高效液相色谱法测定苦参中苦参碱、氧化苦参碱的含量,色谱柱为Angilent NH2(250 mm×4.6 mm,5μm),流动相为乙腈-无水乙醇-3%磷酸溶液(82∶9∶9),柱温为30℃,流速为1.0 mL/min,检测波长220 nm。结果:薄层色谱斑点清晰,分离度较好;高效液相色谱测定苦参碱在0.07030~1.75750μg(r=0.9999)、氧化苦参碱在0.06926~1.73155μg(r=0.9999)范围内线性关系良好,平均回收率分别为101.3%、97.4%,RSD分别为2.0%、1.7%(n=6)。结论:本研究方法准确可靠、重复性好,可用于康妇炎胶囊中苦参的质量控制。Objective To study and control the quality of Sophora flavescens Ait in Kangfuyan capsule.Methods TLC was used for qualitative identification of Sophora flavescens Ait.HPLC was used for content determination of matrine and oxymatrine in Sophora flavescens Ait on a Angilent NH2(250 mm×4.6 mm,5μm),and the mobile phase was acetonitrile-absolute ethanol-3%H 3PO 4(82∶9∶9)at the column temperature of 30℃.The flow rate was 1.0mL/min and the detection wavelength was 220 nm.Results The TLC spots were clear and showed a good separation.HPLC showed the linear range of matrine was 0.07030~1.75750μg(r=0.9999)、HPLC showed the linear range of oxymatrine was 0.06926~1.73155μg(r=0.9999),the average recovery Respectively was 101.3%、97.4%,and the RSD Respectively was 2.0%、1.7%(n=6).Conclusion The method is accurate with good reproducibility,which can be used for the quality control of Sophora flavescens Ait in Kangfuyan capsule.
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