鸡精液稀释配方对低温保存精子活力和输精效果的影响  

Effects of Different Diluents on Sperm Motility and Insemination Results of Chicken Semen in Low-temperature Storage

作　　者：周乐乐 李承德 黄建豪 陈云 曾繁文 黄晓刚 张发良 郑煦灿 严常燕 卫恒习[1] 张守全[1] ZHOU Lele;LI Chengde;HUANG Jianhao;CHEN Yun;ZENG Fanwen;HUANG Xiaogang;ZHANG Faliang;ZHENG Xican;YAN Changyan;WEI Hengxi;ZHANG Shouquan(Animal Genetics and Breeding Laboratory,College of Animal Science,South China Agricultural University,Guangzhou 510642, China;South Sea Breeding Poultry Corporation,Foshan 528225,China)

机构地区：[1]华南农业大学动物科学学院,动物遗传与繁殖实验室,广州510642 [2]佛山市南海种禽有限公司,佛山528225

出　　处：《中国畜牧兽医》2020年第6期1837-1843,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31572397)。

摘　　要：为了完善中国南方地区鸡场公鸡精液的保存技术、提高精液的利用率,本试验研究了在低温(4℃)保存的条件下,不同的保存时间(0、4、8、24 h)、不同的稀释液配方(原精液、配方Ⅰ和配方Ⅱ)对精液的精子活力变化以及人工输精繁殖效果的影响。选用33周龄黄鸡母鸡192只、公鸡42只,192只母鸡依笼号分为12组(3种处理精液×4个保存时间),每组16只母鸡,公鸡不分组。统一采精后用两种不同稀释液稀释、低温(4℃)保存至0、4、8、24 h后观察精子活力,并对母鸡输精,分组收集鸡蛋,对各组受精率、出雏率、健雏率进行比较分析,以原精液低温保存作为对照。结果显示,两种稀释液组的精子低温(4℃)保存4、8、24 h,其精子活力极显著高于原精液组(P<0.01),配方Ⅱ组精子的活力高于配方Ⅰ组(P>0.05);两种配方稀释液组的精液低温(4℃)保存4 h,输精受精率高于原精液组(P<0.05);两种配方稀释液组的精液在低温(4℃)保存8 h,输精受精率极显著高于原精液组(P<0.01)。表明这两种精液稀释液更有利于精液保存,经稀释后的精液可以显著提高受精率,可为中国南方鸡场种公鸡精液保存技术的完善提供有力的数据支撑。For the improvement of the skills of semen preservation and the usage of semen of chicken farms in Southern China,the effects of different storage time(0,4,8,24 h)and different diluents(the original、formulaⅠand formulaⅡ)on sperm motility and the results of artificial insemination in low-temperature(4℃)storage were studied in this trial.192 Huang hens of 33 weeks old were selected and divided into 12 groups as(3 sorts of processed semen×4 storage time)with 16 hens for each,42 Huang cocks of 33 weeks old were selected as one group.After collecting semen,two kinds of diluents was used to dilute semen and kept them in 4℃for 0,4,8,24 hours,then the sperm motility and inseminated were observed,eggs were collected from all groups for measurement of the fertilization rate,birth rate and healthy chicks rate of each group.The experiment was taken with the original semen kept in 4℃as control.The results showed that sperm motility of the two dilute semen was extremely significantly higher than that of the semen group(P<0.01)and the sperm motility of formulaⅡgroup was higher than that of formulaⅠgroup(P>0.05)after storage of 4,8 and 24 h.Fertilization rates of the two dilute semen were significantly higher than that of the semen group(P<0.05)after storage of 4 h;Fertilization rates of the two dilute semen were extremely significantly higher than that of the semen group(P<0.01)after storage of 8 h.The two semen diluents were more effective for semen preservation,and the two dilute semen could significantly improve the fertilization rate.The result provided a powerful support for the improvement of semen preservation skills in chicken farms in Southern China.

关 键 词：精液稀释液 精子活力 受精率 出雏率 健雏率 

分 类 号：S831[农业科学—畜牧学]