献血人群隐匿性乙型肝炎病毒感染的血清学特征分析  

Analysis of HBV serological characteristics in occult HBV infection among blood donors

作　　者：吕莹 伍晓菲[2] 毛日成 贾尧[2] 张继明 王迅[2] LüYing;WU Xiaofei;MAO Richeng;JIA Yao;ZHANG Jiming;WANG Xun(Department of Infectious Diseases,Huashan Hospital,Fudan University,Shanghai 200040,China;Shanghai Blood Center,Shanghai 200051,China;Department of Traditional Chinese Medicine,Shanghai Public Health Center,Fudan University,Shanghai 201508,China)

机构地区：[1]复旦大学附属华山医院感染科,上海200040 [2]上海市血液中心,上海200051 [3]上海市(复旦大学附属)公共卫生临床中心中医科,上海201508

出　　处：《微生物与感染》2020年第3期166-171,共6页Journal of Microbes and Infections

摘　　要：旨在研究献血人群隐匿性乙型肝炎病毒感染(occult hepatitis B virus infection,OBI)的血清学特征。收集上海市血液中心2013-2018年经核酸检测合格血液样本523例,不合格样本395例,分别采用荧光定量聚合酶链反应(fluorescence quantify polymerase chain reaction,FQ-PCR)和转录介导的扩增(transcription mediated amplification,TMA)技术对阳性样本进行HBV DNA鉴定;化学发光法对所有样本HBV表面抗体(抗-HBs)进行定量检测,对HBV e抗原(HBeAg)、HBV e抗体(抗-HBe)和HBV核心抗体(抗-HBc)进行定性检测。结果发现197例HBsAg阴性样本的HBV DNA呈阳性,为OBI。其抗-HBc阳性率为85.79%,抗-HBs阳性、抗-HBc阳性及抗-HBc单独阳性为OBI的主要血清学模式。核酸检测阴性的合格献血者组以乙型肝炎两对半阴性及抗-HBs阳性为主。OBI组抗-HBs水平分布以<100 mIU/mL水平为主,明显低于合格献血者组。合格献血者组>1000 mIU/mL的比例高于OBI组。以上结果表明上海地区献血人群中OBI者血清学分布特征与合格献血者组之间存在差异,为进一步研究OBI提供了数据参考。The purpose of this study is to analyse the hepatitis B virus(HBV)serological characteristics in occult hepatitis B virus infection(OBI)among blood donors.We collected 395 nucleic acid test positive blood samples from 2013 to 2018 in Shanghai Blood Center,which were with normal ALT and with HBsAg,anti-HCV and HIV antigen-antibody negative,and subjected them for HBV DNA identification with PCR or transcription-mediated amplification(TMA)essays.HBV serological markers were tested by chemiluminescent method in all of the samples.We found that among 395 nucleic acid test positive samples,197 were positive for HBV DNA identification,which were considered to be OBI.Most of the OBI samples were positive for anti-HBc(85.79%).The main serological patterns in OBI were anti-HBs positive and anti-HBc positive or anti-HBc positive alone.The main serological patterns in the qualified blood donors were seronegative and anti-HBs positive.The level of anti-HBs in OBI group was mainly below 100 mIU/mL and lower than that in the qualified blood donors(Z=-0.198,P<0.05).It indicated that the HBV serological markers in most of the occult HBV infected people in Shanghai area are different from the qualified blood donors.

关 键 词：乙型肝炎 乙型肝炎病毒 隐匿性乙型肝炎病毒感染 核酸检测 乙型肝炎血清学标志物 合格献血者 

分 类 号：R446.62[医药卫生—诊断学] R512.6+2[医药卫生—临床医学]