不同剂量电离辐射对人外周血淋巴细胞DNA损伤修复相关基因转录水平的影响  

Effects of different dose ionizing radiation on the transcriptional level of DNA damage repair related genes in human peripheral blood lymphocytes

作　　者：陈慧峰[1] 谭斯文 闫雪华[1] 王恰[1] 杨宇华[1] 邹剑明[1] CHEN Huifeng;TAN Siwen;YAN Xuehua;WANG Qia;YANG Yuhua;ZOU Jianming(Guangdong Province Hospital for Occupational Disease Prevention and Treatmen,Guangdong Provincial Key Laboratory of Occupational Diseases Prevention and Treatment,Guangzhou 510300 China;Guangdong Medical University)

机构地区：[1]广东省职业病防治院,广东省职业病防治重点实验室,广东广州510300 [2]广东医科大学

出　　处：《中国辐射卫生》2020年第1期7-12,共6页Chinese Journal of Radiological Health

基　　金：国家自然科学基金(81302387);广东省自然科学基金(2019A1515011969);广东省医学科研基金立项课题(A2017227);广东省职业病防治重点实验室(2017B030314152)。

摘　　要：目的阐明不同剂量电离辐射对人外周血淋巴细胞中DNA损伤修复相关基因(hOGG1、MGMT、PPP2R1A和PPP2R2D)转录水平的影响。方法抽取志愿者外周静脉血约30 mL,分置于7支肝素锂抗凝管中,以剂量率为0.38 mGy·min-1的60Coγ射线(剂量分别为0、0.1、0.2、0.4、0.8、1.6和3.2 Gy)对人外周血照射后培养12 h,分离外周血淋巴细胞,提取总RNA并进行定量和纯度分析。采用实时荧光定量聚合酶链式反应(qRT-PCR)的方法分析不同剂量电离辐射诱导DNA损伤修复相关基因hOGG1、MGMT、PPP2R1A和PPP2R2D基因转录水平的变化。结果 hOGG1基因、MGMT基因和PPP2R2D基因的mRNA转录水平均在0.1 Gy时达到峰值。PPP2R1A基因的mRNA转录水平在0.2 Gy剂量时达到峰值。hOGG1基因转录水平在0.1和0.2 Gy剂量组均较对照组显著增高,差异均具有统计学意义(P <0.05)。MGMT基因转录水平在0.1、0.2和0.8 Gy剂量组均明显高于对照组,差异均具有统计学意义(P <0.05)。PPP2R1A基因转录水平在0.2 Gy剂量组比对照组明显增高,在3.2Gy剂量组比对照组明显降低,差异均具有统计学意义(P <0.05)。PPP2R2D基因转录水平在0.1、0.2、0.4和0.8 Gy剂量组均明显高于对照组,3.2 Gy剂量组比对照组明显降低,差异均具有统计学意义(P <0.05)。结论不同剂量电离辐射均可诱导DNA损伤修复相关的应答基因hOGG1、MGMT、PPP2R1A和PPP2R2D的转录水平改变,适宜剂量的电离辐射(0.1~0.2 Gy)可诱导细胞产生辐射兴奋效应或适应性反应。Objective To investigate the effects of different dose ionizing radiation on the transcriptional level of DNA damage and repair related genes(hOGG1, MGMT, PPP2 R1 A and PPP2 R2 D) in human peripheral blood lymphocytes.Methods 30 mL peripheral blood was extracted from a volunteer and was subpackaged into seven heparin lithium-anticoagulant tubes. Human peripheral blood was irradiated by using 60 Co-γ rays(doses of 0, 0.1, 0.2, 0.4, 0.8, 1.6 and 3.2 Gy) at a dose rate of 0.38 Gy/min. Peripheral blood lymphocytes were isolated after 12 h and then total RNA was extracted for quantitative and purity analysis. Real-time fluorescence quantitative polymerase chain reaction method was used to analyze the changes of transcriptional level of hOGG1, MGMT, PPP2 R1 A and PPP2 R2 D gene. Results The transcription levels of hOGG1, MGMT and PPP2 R2 D gene all reached a peak of 0.1 Gy. The transcription level of PPP2 R1 A reached a peak of0.2 Gy. Compared with the control group, the transcription levels of hOGG1 gene increased significantly at 0.1 Gy and 0.2 Gy(P < 0.05). The transcription levels of MGMT gene at 0.1 Gy, 0.2 Gy and 0.8 Gy were all significantly higher than that in the control group(P < 0.05). Compared with the control group, the transcription levels of PPP2 R1 A gene were increased significantly at 0.2 Gy(P < 0.05). Compared with the control group, the transcription level of PPP2 R1 A gene was decreased significantly at 3.2 Gy(P < 0.05). Compared with the control group, the transcription levels of PPP2 R2 D gene increased significantly at 0.1 Gy 0.2 Gy, 0.4 Gy and 0.8 Gy. Compared with the control group, the transcription level of PPP2 R2 D gene was decreased significantly at 3.2 Gy(P < 0.05). Conclusion Different dose ionizing radiation could induce changes in the transcriptional levels of the genes of DNA damage repair enzyme including, hOGG1, MGMT, PPP2 R1 A and PPP2 R2 D.Suitable low dose ionizing radiation(0.1~0.2 Gy) may induce radiation hormesis or adaptive response of cells.

关 键 词：低剂量电离辐射 DNA损伤修复 8-羟基脱氧鸟嘌呤DNA糖苷酶-1基因 O6-甲基鸟嘌呤-DNA-甲基转移酶基因 蛋白磷酸酶2A-Aα亚基基因 蛋白磷酸酶2A-Bδ亚基基因 

分 类 号：Q691[生物学—生物物理学]