蜱传脑炎病毒感染小鼠脑组织单核细胞趋化蛋白1(MCP-1)、CD68和核因子κB(NF-κB)表达增加  

Tick-borne encephalitis virus(TBEV)enhances the expression of MCP-1,CD68 and NF-κB in infected mouse brain tissue

作　　者：王倩云 彭晖 郑振华[2] 芦宝静 WANG Qianyun;PENG Hui;ZHENG Zhenhua;LU Baojing(Department of Microbiology,School of Basic Medical Science,Anhui Medical University,Hefei 230032;Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China)

机构地区：[1]安徽医科大学基础医学院微生物学教研室,安徽合肥230032 [2]中国科学院武汉病毒研究所,湖北武汉430071

出　　处：《细胞与分子免疫学杂志》2020年第1期9-13,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：十三五传染病重大科技专项(2018ZX10711001-005-002);国家自然科学基金(81401658);2018年度中国科学院高致病性病原生物学与生物安全重点实验室及中国科学院武汉国家生物安全实验室开放研究基金项目(2018SPCAS002);安徽医科大学科研攻关项目(YJGG202007)。

摘　　要：目的探讨蜱传脑炎病毒(TBEV)感染小鼠单核细胞趋化蛋白1(MCP-1)、CD68和核因子κB(NF-κB)表达情况。方法1周龄BALB/c小鼠8只,随机分为对照组和感染组,分别进行PBS或TBEV颅内注射。感染8 d后处死取一半脑组织固定,HE染色检测脑组织病理改变;免疫组织化学染色法检测小鼠大脑MCP-1、NF-κB和CD68的表达,镜下观察并统计阳性细胞数量;免疫荧光化学染色法结合激光扫描共聚焦显微镜观察MCP-1和CD68的共表达情况。结果与对照组相比,TBEV感染后小鼠脑组织MCP-1、NF-κB和CD68阳性细胞数均显著增加,MCP-1在CD68阳性及非阳性细胞中均有表达。结论TBEV感染小鼠CD68、NF-κB、MCP-1表达增强。Objective To investigate the expression of monocyte chemoattractant protein 1(MCP-1),CD68 and nuclear factor kappa B(NF-κB)in mice infected by tick-borne encephalitis virus(TBEV).Methods One-week-old BALB/c mice were randomly divided into control group and infection group with 4 mice in each group.PBS and TBEV was intracranially injected to the control and infection groups,respectively.The mice were sacrificed and half of the brain in each group was fixed after 8 days of infection.HE staining was performed to observe the pathological changes.The expression of MCP-1,NF-κB and CD68 in the brain tissue were detected by immunohistochemical staining and the number of positive cells was counted under a microscope.Immunofluorescence technique combined with laser scanning confocal microscope was used to detect the co-expression of MCP-1 and CD68.Results Compared with the control group,the number of positive cells that expressed MCP-1,NF-κB and CD68 significantly increased in the TBEV infection group and MCP-1 was expressed in both CD68-positive and non-CD68-positive cells.Conclusion TBEV can enhance the expression of MCP-1,CD68 and NF-κB in mouse brain tissue.

关 键 词：蜱传脑炎病毒(TBEV) 单核细胞趋化蛋白1(MCP-1) 核转录因子κB(NF-κB) CD68 

分 类 号：R373.3[医药卫生—病原生物学] R392.1[医药卫生—基础医学]