miRNA-21-5p对食管癌的诊断价值及其与STAT3的相关性研究  

Diagnostic role of miRNA-21-5p for esophageal carcinoma and correlation of STAT3

作　　者：王燕忠[1] 丁丽敏 陈一瑞[2] 王永斌 WANG Yanzhong;DING Limin;CHEN Yirui;WANG Yongbin(Department of Clinical Laboratory,Xiasha Branch,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,Zhejiang,China;Department of Hematology,Zhejiang Provincial People's Hospital,Hangzhou 310014,Zhejiang,China;The Affiliated Hospital of Yangzhou University,Yangzhou 225001,Jiangsu,China)

机构地区：[1]浙江大学医学院附属邵逸夫医院下沙院区检验科,浙江杭州310016 [2]浙江省人民医院血液内科,浙江杭州310014 [3]扬州大学附属医院,江苏扬州225001

出　　处：《检验医学》2020年第6期531-534,共4页Laboratory Medicine

摘　　要：目的分析微小RNA(miRNA)-21-5p在食管癌中的潜在诊断价值,并探索其可能的作用机制。方法选取2016年1月-2018年6月在浙江大学医学院附属邵逸夫医院下沙院区治疗的食管癌患者78例(病例组)以及体检健康者69名(对照组)。采集患者治疗前血液及术中癌组织、癌旁组织,通过实时聚合酶链反应(PCR)检测血清中miRNA-21-5p的表达,通过picTar、TagetScan、Tarbase 3个数据库分别预测miRNA-21-5p的靶基因;利用Draw venn diagram选取3个数据库预测靶基因交集;采用实时PCR检测食管癌患者癌组织和癌旁组织中信号传导和转录激活子3(STAT3)mRNA的表达情况。结果实时PCR结果显示,miRNA-21-5p在食管癌患者血清中高表达,且显著高于对照组(P<0.01);STAT3 mRNA在食管癌患者癌组织中高表达,显著高于癌旁组织(P<0.01);Spearman相关性分析发现两者呈正相关。结论在食管癌患者血清中miRNA-21-5p呈高表达,同时在癌组织中miRNA-21-5p的预测靶基因STAT3 mRNA也呈高表达,并且两者呈正相关。Objective To evaluate the diagnostic role of microRNA(miRNA)-21-5p for esophageal carcinoma and its mechanism.Methods A total of 78 patients with esophageal carcinoma from January 2016 to June 2018 in Xiasha Branch,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine were enrolled as case group,and 69 healthy subjects were enrolled as control group.Blood and esophageal carcinoma and adjacent tissues were collected before treatment and during operation.The expression of miRNA-21-5p in serum was determined by real-time polymerase chain reaction(PCR).The target gene of miRNA-21-5p was predicted by 3 databases,including picTar,TagetScan and Tarbase.The target gene intersection of the 3 databases was predicted using Draw venn diagram.Real-time PCR was used to determine the expression of signal transducer and activator of transcription 3(STAT3)mRNA in esophageal carcinoma and adjacent tissues in patients with esophageal carcinoma.Results The results of real-time PCR showed that miRNA-21-5p was highly expressed in serum of patients with esophageal carcinoma,which was higher than that in control group(P<0.01).The high expression of STAT3 mRNA was also found in esophageal carcinoma tissues of patients with esophageal carcinoma,which was higher than that in adjacent tissues.Spearman correlation analysis showed that miRNA-21-5p and STAT3 were positively correlated.Conclusions The expression of miRNA-21-5p in serum of patients with esophageal carcinoma is high,and STAT3 mRNA is also highly expressed in esophageal carcinoma tissues.There is a positive correlation between miRNA-21-5p and STAT3.

关 键 词：微小RNA-21-5p 食管癌 诊断价值 

分 类 号：R446.1[医药卫生—诊断学]