坐骨神经分支选择损伤小鼠腰段脊髓背角spalt样转录因子1(Sall1)表达下调且炎症因子表达增强  

Down-regulated expression of Sall1 and enhanced expression of inflammatory factors in dorsal horn of spinal cord of mice with sciatic nerve branch selective injury

作　　者：陆培春 张进[2] 徐静[2] 蒋锦 孙彩霞[2] 吴进 苏兆亮[3] LU Peichun;ZHANG Jin;XU Jing;JIANG Jin;SUN Caixia;WU Jin;SU Zhaoliang(Medical College,Jiangsu University,Zhenjiang 212000,China;Department of Anesthesiology,Affiliated Hospital,Jiangsu University,Zhenjiang 212000,China;Institute of Immunology,Jiangsu University,Zhenjiang 212000,China)

机构地区：[1]江苏大学医学院,江苏镇江212000 [2]江苏大学附属医院麻醉科,江苏镇江212000 [3]江苏大学免疫学研究所,江苏镇江212000

出　　处：《细胞与分子免疫学杂志》2020年第2期111-116,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：江苏省“六大人才”高峰项目(019-WSN-122)。

摘　　要：目的探讨spalt样转录因子1(Sall1)在坐骨神经分支选择损伤的神经病理性疼痛模型小鼠腰段脊髓背角中的表达及定位。方法6~8周龄BALB/c小鼠随机分成对照组、假手术组和坐骨神经分支选择损伤组(SNI组)。在模型建立之前的1 d及术后第3、7、10、14天,测定小鼠机械缩足反应阈值(MWT);分别于手术建模后3、7、10、14 d,收集小鼠L4~6节段的患侧脊髓背角部分,反转录PCR检测Sall1 mRNA表达及术后7 d炎症因子白细胞介素1β(IL-1β)、IL-6、肿瘤坏死因子α(TNF-α)的mRNA水平;Western blot法测定上述4个时间点的Sall1蛋白含量,免疫组织化学染色法检测术后3、7、10、14 d脊髓Sall1的表达定位并进行半定量分析。结果与对照组、假手术组相比,SNI组术后3、7、10、14 d,MWT均下降;与对照组相比,SNI组Sall1 mRNA和蛋白水平在术后7、10、14 d均下降,术后7 d,IL-1β、IL-6、TNF-αmRNA水平增加;对照组、SNI组Sall1主要定位于脊髓背角,且术后除3 d外各时间点SNI组的含量明显低于对照组。结论Sall1定位于脊髓背角,坐骨神经分支选择损伤可致患侧腰段脊髓背角的Sall1表达下降,IL-1β、IL-6、TNF-αmRNA表达增加。Objective To investigate the expression and distribution of spalt-like transcription factor 1(Sall1)in the lumbar spinal dorsal horn of mice with sciatic nerve branch selective injury.Methods BALB/c mice aged 6~8 weeks old were randomly divided into a control group,a sham operation group and a sciatic nerve branch selective injury group.The mechanical withdrawal threshold(MWT)was measured on the 1st day before the establishment of the model and on the 3rd,7th,10th and 14th day after operation.On the 3rd,7th,10th and 14th day after operation,the dorsal horn was collected from the L4-6 segments of the affected spinal cord.The expression of Sall1 and the mRNA levels of inflammatory cytokines such as interleukin 1β(IL-1β),IL-6 and tumor necrosis factor alpha(TNF-α)on the 7th day after operation were detected by real-time quantitative PCR.The expression of Sall1 protein was determined by Western blot analysis at the above four time points.Immunohistochemical staining and semi-quantitative analysis were performed to analyze the expression and distribution of Sall1 in the spinal cord on the 3rd,7th,10th and 14th day after operation.Results Compared with the control group and the sham operation group,MWT of the SNI group decreased on the 3rd,7th,10th and 14th day after operation.Compared with the control group,the mRNA and protein levels of Sall1 in SNI group decreased on the 7th,10th and 14th day after operation.The mRNA levels of IL-1β,IL-6 and TNF-αincreased at 7th day after operation.Sall1 in the control group and SNI group was mainly distributed in the spinal dorsal horn,and the expression in the SNI group was significantly lower than that in the control group at all time points except for the 3th day after operation.Conclusion Sall1 is distributed in the dorsal horn of spinal cord.Sciatic nerve branch selective injury could decrease the expression of Sall1 and increase the expression of IL-1β,IL-6 and TNF-αmRNA in the dorsal horn of lumbar spinal cord.

关 键 词：spalt样转录因子1(Sall1) 神经病理性疼痛 白细胞介素1β(IL-1β) IL-6 肿瘤坏死因子α(TNF-α) 

分 类 号：R392.11[医药卫生—免疫学] R392-33[医药卫生—基础医学]