Fecal microbiota transplantation improves metabolism and gut microbiome composition in db/db mice  

作　　者：Pei-pei Zhang Lin-lin Li Xue Han Qin-wei Li Xu-hua Zhang Johnson J.Liu Ye Wang 

机构地区：[1]Department of Pharmacology,Xinjiang Medical University,Urumqi 830000,China [2]State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia of Xinjiang Medical University,Urumqi 830000,China [3]Department of Otorhinolaryngology,Heze Municipal Hospital,Heze 274031,China [4]Department of Pharmacology,School of Medical Sciences,Faculty of Medicine,University of New South Wales,Sydney,NSW Australia

出　　处：《Acta Pharmacologica Sinica》2020年第5期678-685,共8页中国药理学报（英文版）

基　　金：the National Natural Science Foundation of China(grant No.81360504);the NSFC–Xinjiang Joint Fund(U1403322)for Excellent Young Scholars.We also thank the Kazaks donor for contributing to this study and the Experimental Animal Center of Xinjiang Medical University for their kindly assistance.

摘　　要：Fecal microbiota transplantation(FMT)has become an effective strategy to treat metabolic diseases,including type 2 diabetes mellitus(T2DM).We previously reported that the intestinal microbiome had significant difference between individuals with normal glucose tolerance and T2DM in Chinese Kazak ethnic group.In this study,we investigated the effects of transplanted fecal bacteria from Kazaks with normal glucose tolerance(KNGT)in db/db mice.The mice were treated with 0.2 mL of fecal bacteria solution from KNGT daily for 10 weeks.We showed that the fecal bacteria from KNGT successfully colonized in the intestinal tract of db/db mice detected on day 14.In the FMT-treated db/db mice,the levels of fasting blood glucose,postprandial glucose,total cholesterol,triglyceride,and low-density lipoprotein–cholesterol were significantly downregulated,whereas high-density lipoprotein–cholesterol levels were upregulated.In the FMT-treated db/db mice,Desulfovibrio and Clostridium coccoides levels in gut were significantly decreased,but the fecal levels of Akkermansia muciniphila and colon histone deacetylase-3(HDAC3)protein expression were increased.At 8 weeks,both intestinal target bacteria and HDAC3 were correlated with glycolipid levels;Akkermansia muciniphila level was positively correlated with HDAC3 protein expression(r=+0.620,P=0.037).Our results suggest that fecal bacteria from KNGT could potentially be used to treat diabetic patients.

关 键 词：fecal microbial transplantation Chinese Kazak ethnic group metabolic diseases db/db mice intestinal microbiome DESULFOVIBRIO Clostridium coccoides Akkermansia muciniphila colon histone deacetylase-3 

分 类 号：R96[医药卫生—药理学]