摩西管柄囊霉(Funneliformis mosseae)对连作大豆根际土壤细菌菌群的影响  

Effect of Funneliformis mosseae on Bacterial Flora in Continuous Rhizosphere Soil of Soybean

作　　者：崔晓莹 白莉[3] 郭娜[3] 殷继忠 蔡柏岩[1,2] CUI Xiao-ying;BAI Li;GUO Na;YIN Ji-zhong;CAI Bai-yan(College of Life sciences,Heilongjiang University,Harbin 150080,China;Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region,Heilongjiang University,Harbin 150080,China;Department of Food and Environmental Engineering,Heilongjiang Oriental College,Harbin 150086,China)

机构地区：[1]黑龙江大学生命科学学院,黑龙江哈尔滨150080 [2]黑龙江省寒地生态修复与资源利用重点实验室,黑龙江哈尔滨150080 [3]黑龙江东方学院食品与环境工程学部,黑龙江哈尔滨150066

出　　处：《大豆科学》2020年第2期277-287,共11页Soybean Science

基　　金：国家自然科学基金(31972502)。

摘　　要：为探究丛枝菌根真菌(Arbuscula mycorrhizal fungi,AMF)-摩西管柄囊霉(Funneliformis mosseae)对不同连作年限大豆土壤细菌菌群的影响,分析了接种F.mosseae后连作0,1,3和5年的根系菌根侵染率、细菌菌群结构和多样性以及KEGG功能预测,从而为AMF缓解大豆连作障碍提供理论依据。结果表明:大豆土壤细菌菌群多样性和丰度随着连作年限升高而减少,鞘氨醇单胞菌属(Sphingomonas)、芽单胞菌属(Gemmatimonas)、芽孢杆菌属(Bacillus)和硝化杆菌属(Nitrobacter)等有益菌丰度也随着连作年限升高而减少。而接种F.mosseae不仅能改善根际土壤的细菌多样性,提高Sphingomonas、Gemmatimonas、Nitrospira和Lysobacter等有益菌的丰度,还改善了根际细菌群落氨基酸代谢、强化信号转导、膜转运和碳水化合物代谢等功能,显著缓解了大豆连作障碍。In order to investigate the effect of arbuscula mycorrhizal fungi(AMF)-Funneliformis Mosseae on the bacterial community in soybean soil after different continuous cropping years, we analyzed the infection rate, bacterial community structure and diversity of AMF after 0,1,3,5 continuous cropping years respectively, and KEGG function prediction was also conducted. And we hoped this study could provides theoretical basis for alleviating soybean continuous cropping obstacle. The results showed that, with the increase of continuous cropping years, the diversity and abundance of bacteria in soybean soil, and the abundance of beneficial bacteria such as Sphingomonas, Gemmatimonas, Bacillus and Nitrobacter were also decreased. Inoculation of F. mosseae not only improved the bacterial diversity in rhizosphere soil, increased the abundance of beneficial bacteria such as Gemmatimonas, Sphingomonas, Nitrospira and Lysobacter, but also improved the functions of amino acid metabolism, signal transduction, membrane transport and carbohydrate metabolism of rhizosphere bacterial community, which significantly alleviated the soybean continuous cropping obstacle.

关 键 词：连作大豆 摩西管柄囊霉 土壤细菌菌群 KEGG功能预测 

分 类 号：S565.1[农业科学—作物学]