猪伪狂犬病病毒山西分离株gE全基因的遗传变异多样性及流行特点分析  

Epidemic characteristic and analysis of genetic variation diversity of gE gene of pseudorabies virus isolated from Shanxi area

作　　者：孟帆[1] 樊振华[1] 吴忻[1] 姚敬明[1] 韩一超[1] 薛翼鹏[1] 李红丽[1] 米瑞娟[1] 赵岳[1] MENG Fan;FAN Zhen-hua;WU Xin;YAO Jing-ming;HAN Yi-chao;XUE Yi-peng;LI Hong-li;MI Rui-juan;ZHAO Yue(Institution of Animal Husbandry and Veterinary,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China)

机构地区：[1]山西省农科院畜牧兽医研究所,山西太原030032

出　　处：《中国兽医学报》2020年第5期873-881,共9页Chinese Journal of Veterinary Science

基　　金：山西省科技攻关资助项目(201603D221023-1)。

摘　　要：为了研究当前山西省猪伪狂犬病(PR)的流行特点及遗传变异情况,对山西分离的3株猪伪狂犬病病毒(PRV)分离株(SXQX株、SXTG株和SXYP株)的gE全基因进行了扩增、克隆和测序(已被GenBank收录)。并将gE全基因序列和推导出的氨基酸序列与不同国家和地区的主要流行株进行了遗传变异多样性分析。结果显示,3株PRV分离株的gE全基因序列之间核苷酸及推导的氨基酸序列同源性分别为99.7%~100.0%和99.5%~100.%,与34株不同国家和地区的PRV参考株之间同源性分别为97.1%~100.0%和94.6%~100.0%,与欧美分离株的同源性分别为97.1%~97.6%和94.6%~95.7%,与2012年以来我国不同省份分离株的同源性分别为98.8%~100.0%和98.1%~100.0%,与2012年以前分离的经典毒株同源性分别为98.4%~99.7%和97.2%~99.3%。PRV gE全基因组序列的进化分析表明,3株PRV分离株属于亚洲基因群中2012年以来国内不同地区相继分离的PRV变异株群,而与2012年前分离的经典强毒株群亲缘关系较远,与欧美基因群亲缘关系最远。进一步进行核苷酸及氨基酸多序列比对分析发现,3株PRV分离株的gE基因均有一些位点发生了突变。本试验为近年来山西地区PRV的流行特点和PRV变异情况提供了依据。In order to study the epidemic statue and the genetic variation diversity of pseudorabies virus(PRV) strains in Shanxi.The genomic sequences of three PRV strains SXQX,SXTG and SXYP which were isolated recently from some areas of Shanxi were cloned,sequenced and received by GenBank.The amplified gE genomic sequences and deduced amino acid sequences of the three PRV strains were analysed and compared with that of published PRV stains by DNAStar,drawing phylogenetic tree.The results showed that the homologies of nucleotide and amino acid sequences of gE gene of the three strains were 99.7%-100.0% and 99.5%-100.0% respectively,the homologies of nucleotide and amino acid sequences of the three strains to the 34 isolates from different regions of the world PRV strain were 97.1%-100.0% and 94.6%-100.0% respectively,the homologies of nucleotide and amino acid sequences of the three strains to the Euramerican stains were 97.1%-97.6% and 94.6%-95.7% respectively,the homologies of nucleotide and amino acid sequences of the three strains to China PRV strains from different regions since 2012 were 98.8%-100.0% and 98.1%-100.0% respectively,and the homologies of nucleotide and amino acid sequences of the three strains to China PRV strains from different regions before 2012 were 98.4%-99.7% and 97.2%-99.3% respectively.The phylogenetic of gE genomic sequences showed that the three strains belonged to the variation isolates from different regions of China PRV strains since 2012 in Asian genetic group,but were far with the classic strain groups before 2012,and the close with the Euramerican genetic group.Further the analysis of nucleotide sequence and amino acid sequence alignment revealed that some of gE loci of the three PRV isolates were mutated.The results provided a theoretical basis for the study of recent PRV epidemic characteristic and variation in Shanxi area.

关 键 词：猪伪狂犬病病毒 流行特点 gE全基因 遗传变异 多样性分析 

分 类 号：S852.65[农业科学—基础兽医学]