囊性包虫病患者外周血PD-1在Treg细胞上的表达与Foxp3、TGF-β和IL-10的相关性研究  

Study on the correlation between the expression of PD-1on Treg cells of peripheral blood and Foxp3,TGF-beta and IL-10 in patients with cystic echinococcosis

作　　者：赵慧[1] 张峰波[1] 朱玥洁[1] 庞楠楠[1] 安梦婷 李斌[2] 马秀敏[1] 丁剑冰[1,2] ZHAO Hui;ZHANG Feng-bo;ZHU Yue-jie;PANG Nan-nan;AN Meng-ting;LI Bin;MA Xiu-min;DING Jian-bing(The first affiliated hospital,Xinjiang MedicalUniversity,Urumqi 830011,China;Collage of Basic medicine,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学第一附属医院,乌鲁木齐830011 [2]新疆医科大学基础医学院,乌鲁木齐830011

出　　处：《中国人兽共患病学报》2020年第6期475-480,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(Nos.81460307,81660343,81760372);新疆维吾尔自治区自然科学基金资助项目(No.2016D01C325)。

摘　　要：目的通过检测囊性包虫病(cystic echinococcosis,CE)患者外周血中Treg细胞表面PD-1的表达与Treg细胞相关因子Foxp3、TGF-β和IL-10的表达情况,探讨负性共刺激分子PD-1与Treg细胞在CE患者中的关系及其对该疾病发生发展的作用。方法选取30例CE患者和30例健康对照人群,用流式细胞术检测CE患者和健康对照人群外周血中PD-1^+CD4^+CD25^+Treg细胞的比例;实时荧光定量PCR法分别检测CE患者和对照人群外周血单个核细胞中PD-1和Foxp3 mRNA的表达;ELISA法检测CE患者和对照人群血清中TGF-β和IL-10的水平;Pearson相关法分析CE患者外周血中PD-1的表达与Foxp3、TGF-β和IL-10的相关性。结果与对照人群相比,CE患者外周血中PD-1^+CD4^+CD25^+Treg细胞的比例均显著升高;外周血单个核细胞中PD-1和Foxp3 mRNA水平显著升高;血清中TGF-β和IL-10水平显著升高;CE患者外周血PD-1mRNA与Foxp3 mRNA表达水平正相关;PD-1^+CD4^+CD25^+Treg细胞与TGF-β和IL-10的水平均正相关。结论负性共刺激分子PD-1在Treg细胞上高表达与Treg细胞及其因子TGF-β和IL-10的产生和功能发挥相关,可能促进细粒棘球蚴的生长发展。To investigate the relationship between negative costimulatory molecule PD-1 and Treg cells in cystic echinococcosis and their roles in the occurrence and development of the disease,by detecting the expression of PD-1 on Treg cells and Treg cell-related factors Foxp3,TGF-beta and IL-10 in peripheral blood of patients with cystic echinococcosis.Flow cytometry was used to detect the proportion of PD-1^+CD4^+CD25^+Treg cells in peripheral blood of 30 patients with cystic echinococcosis and 30 healthy controls.The expressions of PD-1 and Foxp3 in peripheral blood mononuclear cells were detected by real-time fluorescence quantitative PCR.The serum levels of TGF-beta and IL-10 were detected by ELISA and the correlation between the expression of PD-1 and Foxp3,TGF-beta and IL-10 in peripheral blood of patients with cystic echinococcosis was analyzed by Pearson correlation method.Results showed that comparing with the control group,the proportion of PD-1^+CD4^+CD25^+Treg cells in peripheral blood of patients with cystic echinococcosis increased significantly.The levels of PD-1 and Foxp3 in peripheral blood mononuclear cells increased significantly,while the levels of TGF-beta and IL-10 in serum increased significantly.The expressions of PD-1 and Foxp3 in peripheral blood of patients with cystic echinococcosis were positively correlated,while the levels of TGF-beta and IL-10 in PD-1^+CD4^+CD25^+Treg cells were positively correlated.In conclusion,the high expression of negative costimulatory molecule PD-1 in Treg cells is related to the production and function of Treg cells and cytokines TGF-beta and IL-10,which may promote the sustained development of Echinococcus granulosus infection.

关 键 词：PD-1 Treg 囊性包虫病 

分 类 号：R392.9[医药卫生—免疫学]