一例鸭疫里默氏杆菌病的实验室诊断  

A Case of Laboratory Diagnosis of Infectious Serositis of Duck

作　　者：刘丽娟 马光强 吴良涛 杨均[2] 张海莉 刘军泽 陈国权 王开功[3] 周碧君[3] Liu Lijuan;Ma Guangqiang;Wu Liangtao;Yang Jun;Zhang Haili;Liu Junze;Chen Guoquan;Wang Kaigong;Zhou Bijun(Agriculture and Rural Bureau of Duyun City,Guizhou Province,Duyun Guizhou 558000,China;Qiannan Vocational College of Nationalities,Duyun Guizhou 558000,China;College of Animal Science,Guizhou University,Guiyang Guizhou 550025,China)

机构地区：[1]贵州省都匀市农业农村局,贵州都匀558000 [2]黔南民族职业技术学院,贵州都匀558000 [3]贵州大学动物科学学院,贵州贵阳550025

出　　处：《贵州畜牧兽医》2020年第3期49-51,共3页Guizhou Journal of Animal Husbandry & Veterinary Medicine

摘　　要：为确诊贵州省三穗县某养鸭场疑似鸭疫里默氏杆菌感染病例,通过实验室细菌分离培养、生化试验、PCR检测及基因测序对病原进行鉴定,并进行药物敏感性试验。结果:分离细菌为革兰氏阴性杆菌;生化特征与鸭疫里默氏杆菌相符;PCR检测及基因测序与鸭疫里默氏杆菌OmpA基因同源性达100%;药敏试验对卡那霉素、新霉素、哌拉西林3种抗菌素高度敏感。结论:确诊病例为鸭疫里默氏杆菌病,可根据药敏试验结果选择高敏药物进行防治。In order to confirm the suspected case of Riemerella anatipestifer infection in a duck farm in Sansui county,Guizhou province,the pathogens were identified through laboratory bacterial isolation and culture,biochemical tests,PCR testing and gene sequencing,and drug sensitivity tests were conducted.Results:The isolated bacteria were Gram-negative bacilli;the biochemical characteristics were consistent with Riemerella anatipestifer;PCR detection and gene sequencing were 100%homologous to the OmpA gene of Riemerella anatipestifer;3 antibiotics,neomycin,piperacillin are highly sensitive.Conclusion:the confirmed case is Riemerella anatipestifer,and high-sensitivity drugs can be selected for prevention and treatment according to the results of drug susceptibility tests.

关 键 词：鸭疫里默氏杆菌 细菌分离鉴定 PCR检测 药敏试验 

分 类 号：S858.32[农业科学—临床兽医学]