玉米耐深播主效QTL qMES20-10的精细定位及差异表达基因分析  

Fine mapping of a major QTL qMES20-10 associated with deep-seeding tolerance in maize and analysis of differentially expressed genes

作　　者：任蒙蒙 张红伟[1] 王建华[2] 王国英[1] 郑军[1] REN Meng-Meng;ZHANG Hong-Wei;WANG Jian-Hua;WANG Guo-Ying;ZHENG Jun(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]中国农业大学农学院,北京100193

出　　处：《作物学报》2020年第7期1016-1024,共9页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2016YFD0101002);中国农业科学院创新工程专项经费资助。

摘　　要：干旱是影响玉米(Zea mays L.)产量最主要的环境因素之一,具有耐深播特性的玉米种质材料能够吸收土壤深层水分,具有较强的耐旱性,因此研究玉米耐深播性状的遗传机制具有重要的理论和应用价值。本实验室前期已利用耐深播玉米自交系3681-4与普通自交系X178构建的F2:3群体,在玉米10号染色体上定位到了一个耐深播主效QTLqMES20-10。本研究在此基础上,以X178为轮回亲本,结合前景选择和背景选择,构建了BC3F3:4家系,对qMES20-10迚行了确证;并进一步利用分子标记辅助选择构建了高代回交群体,将其精细定位于133.3~136.0Mb的区间之内。同时,利用从BC3F3:4家系中筛选出的两个近等基因系,迚行差异表达基因分析,发现差异表达基因主要参与了化学性应激反应、氧化还原反应和对氧化胁迫的应激反应。本研究结果为迚一步兊隆耐深播主效QTL qMES20-10奠定了基础。Drought stress is a major threat to maize(Zea mays L.)yield.Deep-seeding tolerant maize variety can absorb water in deep soil and thus have strong drought tolerance.Therefore,it is of great theoretical and practical importance to study the genetic mechanism of deep-seeding tolerance.In our previous work,we identified a major QTL qMES20-10 controlling maize deep-seeding tolerance on chromosome 10 using an F2:3 population derived from a deep-seeding tolerant inbred line 3681-4 and a common inbred line X178.In this study,a BC3F3:4 population was constructed through background and foreground selection using X178 as the recurrent parent.The major QTL,qMES20-10,was firstly verified in this BC3F3:4 population.Furthermore,advanced backcross population was constructed through marker-assisted selection,and qMES20-10 was fine-mapped within the interval of 133.3–136.0 Mb on chromosome 10.Moreover,RNA-Seq analysis of two near-isogenic lines screened from the BC3F3:4 families identified the differentially expressed genes,mainly involved in chemical stimulus response,oxidation reduction,and oxidative stress response.These results lay a foundation of further cloning the major QTL qMES20-10.

关 键 词：玉米 耐深播 QTL 精细定位 差异表达基因 

分 类 号：S513[农业科学—作物学]