大豆品种郑97196抗疫霉病基因RpsZheng精细定位  

Fine mapping of Phytophthora resistance gene RpsZheng in soybean cultivar Zheng 97196

作　　者：张雪翠 钟超 段灿星[1] 孙素丽[1] 朱振东[1] ZHANG Xue-Cui;ZHONG Chao;DUAN Can-Xing;SUN Su-Li;ZHU Zhen-Dong(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Agricultural College,Shenyang Agricultural University,Shenyang 110866,Liaoning,China)

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]沈阳农业大学农学院,辽宁沈阳110866

出　　处：《作物学报》2020年第7期997-1005,共9页Acta Agronomica Sinica

基　　金：国家公益性行业(农业)科研专项经费项目(201303018);农业农村部农作物种质资源保护与利用专项(2019NWB036-12);中国农业科学院科技创新工程项目资助。

摘　　要：大豆疫霉病是由大豆疫霉引起的一种重要大豆病害,可造成严重的经济损失。种植含有抗疫霉病基因的大豆品种是控制该病害最有效的途径。前人在大豆品种郑97196的3号染色体上鉴定了一个抗疫霉病基因RpsZheng。本研究的目的是验证并精细定位抗疫霉病基因RpsZheng。以Williams和郑97196杂交衍生的188个F2:3家系为作图群体,用大豆3号染色体上的SSR标记构建RpsZheng遗传连锁图,获得与RpsZheng紧密连锁的侧翼SSR标记SattWM82_39(2.5 cM)和BARCSOYSSR_03_0269(1.0 cM)。基于亲本间全基因组重测序数据鉴定和开发多态性InDel标记,进一步将RpsZheng候选区域缩小至105.2 kb,通过检测RpsZheng候选区域内的共分离标记特异性,获得了能够有效检测RpsZheng的分子标记WZInDel11。本研究明确了RpsZheng的候选基因组区间,鉴定出了能够有效用于基因功能研究和辅助选择育种的共分离分子标记。Phytophthora root rot caused by Phytophthora sojae,is a major disease in soybean production,which can cause serious yield losses.To date,the most effective way to control the disease is deployment of resistant soybean cultivars containing Phytophthora sojae(Rps)resistance genes.The previous study identified an Rps gene RpsZheng in soybean cultivar Zheng 97196,which was mapped on chromosome 3.The objective of this study was to confirm and finely map the resistance gene RpsZheng.The susceptible Williams and resistant Zheng 97196 were crossed and generated 188 F2:3 families,which were used as a mapping population.The genetic linkage map of RpsZheng was constructed using genetic and phenotypic data.RpsZheng was mapped between the flanking SSR markers SattWM82_39(2.5 cM)and BARCSOYSSR_03_0269(1.0 cM).Based on genome-wide resequencing data of parents,we developed and identified polymorphic InDel markers to further narrow the RpsZheng candidate region to 105.2 kb.Molecular detection among soybean cultivars was carried out using the co-segregation markers in the RpsZheng candidate region.The marker WZInDel11 could effectively distinguish RpsZheng from other Rps genes.This study identified specific genomic intervals of RpsZheng and developed co-segregation markers that can be effectively used for gene function studies and molecular assisted selection breeding.

关 键 词：大豆 大豆疫霉病 抗性遗传分析 抗病基因 精细定位 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]