高效液相色谱法测定化妆品中水杨酸的不确定度评定  

Evaluation of Uncertainty in the Determination of Salicylic Acid in Cosmetics by High Performance Liquid Chromatography

作　　者：张慧敏 彦培傲 徐志杰 鞠玲燕 Zhang Huimin;Yan Peiao;Xu Zhijie;Ju Lingyan(Shandong Marine Economic Algae Resources Development and Utilization Engineering Technology Co-innovation Center,Weihai 264300,China;Department of Marine Biology and Medicine,Weihai Ocean Vocational College,Weihai 264300,China;Weihai Entry-Exit Inspection and Quarantine Bureau,Weihai 264205,China)

机构地区：[1]山东省海洋经济藻类资源开发与利用工程技术协同创新中心,山东威海264300 [2]威海海洋职业学院海洋生物与医药系,山东威海264300 [3]威海出入境检验检疫局检验检疫技术中心,山东威海264205

出　　处：《山东化工》2020年第10期91-95,共5页Shandong Chemical Industry

基　　金：原国家质检总局科技项目(2017IK223)。

摘　　要：对高效液相色谱法检测化妆品中水杨酸的不确定度进行评定。按照《化妆品安全技术规范》(2015年版)规定方法检测化妆品中的水杨酸含量,样品在甲醇+水(75+25)溶液中进行超声提取,经色谱柱分离,流动相梯度洗脱后,用二极管阵列检测器检测,采用外标法定量。依据JJF1059.1-2012《测量不确定度评定与表示》,分析水杨酸测定过程中不确定度的分量及其来源,并通过计算各分量的不确定度得出检测结果的合成不确定度。当化妆品中水杨酸含量为40μg/g时,在95%的置信区间下,扩展不确定为±4.3046μg/g(k=2)。结果表明,不确定度主要来源于标准溶液配置,仪器重复测量,标准曲线的拟合过程等。Base on safety and technical standards of cosmetics(2015 edition),the uncertainty of determination of salicylic acid in cosmetics was evaluated by high performance liquid chromatography(HPLC).The sample was extracted by methanol:water(75∶25)with a gradient elution.The content of salicylic acid in cosmetics was quantitatively determined by using diode array detector and external standard method.According to JJF 1059-2012 Measurement Technical Standard,The components and sources of the uncertainty in the determination of salicylic acid were analyzed.When the salicylic acid content in cosmetics is 40μg/g,the expansion uncertainty is±4.3046μg/g(k=2)at the 95%confidence interval.The evaluation results show that the uncertainty in the experimental process mainly comes from the standard solution configuration,repeated measurement of the instrument,and the fitting process of the standard curve.

关 键 词：高效液相色谱 化妆品 水杨酸 不确定度 

分 类 号：O657.7[理学—分析化学]