清痹片质量标准研究  

Quality Standard Research of Qingbi Tablet

作　　者：陈清波 CHEN Qingbo(Tai'an City Taishan District Center for Disease Control and Prevention,Tai'an,Shandong,271000,China)

机构地区：[1]泰安市泰山区疾病预防控制中心,山东泰安271000

出　　处：《检验检疫学刊》2020年第3期48-50,共3页Journal of Inspection and Quarantine

摘　　要：为了建立清痹片(含金银花、羌活、川牛膝、蒲公英、薏苡仁等9味药)的质量标准,本文采用薄层色谱法(TLC)对清痹片中的金银花和羌活进行定性鉴别,用高效液相色谱法(HPLC)对清痹片中的绿原酸进行含量测定。结果显示,TLC斑点清晰,专属性强,可以检出金银花与羌活中的特征斑点,分离度高。绿原酸在31~155μg/ml(r2=0.99991)范围内呈现良好的线性关系,平均加样回收率(n=6)为96.84%,相对标准偏差(RSD)为3.10%。由此可知,该方法操作简便,重复性好,可有效控制清痹片的质量。In order to establish the quality standard of qingbi tablets(such as lonicerae japonicae,notopterygium root,radix cyrandiae,dandelion,coix seed and so on,about 9 herbs),the TLC method is used to identify the lonicerae japonicae and notopterygium root in qingbi tablets,and the HPLC method is used to identify the chlorogenic acid in qingbi tablets.The results show that the TLC spots are clear and specific,which can detect characteristic spots in lonicerae japonicae and notopterygium root with high degree of separation.The linear range of chlorogenic acid is measured by HPLC within 31—155μg/mL,and the average recovery is 96.84%and RSD is 3.10%(n=6).In conclusion,the method is simple and reproducible,which can be used for the quality control of qingbi tablet effectively.

关 键 词：清痹片 薄层色谱法 绿原酸 高效液相色谱法 

分 类 号：R29[医药卫生—民族医学]