Effect of Gubi prescription on caveolin-1 expression and phosphoinositide 3 kinase/protein kinase B and Fas signal pathways in rats with knee osteoarthritis  

作　　者：He Xiaojin Wang Lei Zhou Xueping Xu Luzhou Cao Jing Wang Ruirui Wang Min Xie Guoqian 

机构地区：[1]Rheumatology Department,Affiliated Hospital of Nanjing University of Chinese Medicine,Jiangsu Province Hospital of Chinese Medicine,Nanjing 210029,China [2]Sports Medicine Center,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China [3]Department of Traumatology&Orthopedics,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China

出　　处：《Journal of Traditional Chinese Medicine》2020年第2期224-235,共12页中医杂志（英文版）

基　　金：Supported by Grants from the Training Project for Young and Middle-aged Academic Leaders of"Jiangsu 333 Project"and"High Level Health Personnel Six One Project"of the Jiangsu Health Planning Commission Study on the Molecular Mechanism of Gubi Prescription in the Treatment of KOA Based on the Effect of Caveolin-1 on Chondrocyte Apoptosis Related Signal Transduction Pathway(LGY2016014);Research Project for Clinical Talents of Chinese Medicine of Jiangsu Province。

摘　　要：OBJECTIVE: To investigate the effects of Gubi prescription on the expression of caveolin-1, and the phosphoinositide 3 kinase/protein kinase B(PI3 K/Akt) and Fas signal pathways in rats with knee osteoarthritis(KOA).METHODS: Forty KOA model rats were established using a modification of Hulth's method. Rats were divided into five groups by the random number method: model, positive drug(Vicolli group), and high-, medium-, and low-dose Gubi prescription groups(n = 8/group). In the sham surgery group(n = 8), only anterior and posterior cruciate ligaments of rats were exposed during surgery. A normal group(n = 8) consisted of rats with no treatment. Rats were intragastrically administered corresponding drugs once every day for eight consecutive weeks. Then, rat synovial membranes were extracted and histomorphological changes were recorded. m RNA expression was measured by q-PCR.Serum superoxide dismutase(SOD), malondialdehyde(MDA), nitric oxide(NO), and interleukin 1β(IL-1β) levels were measured. Western blotting determined the effects of Gubi prescription on protein expressions of caveolin-1, Bax, Bcl-2, Fas, and caspase-3 in chondrocytes from KOA rats. The knee cartilage of rats was excised and cultured under aseptic conditions. After coincubation of chondrocytes with Gubi prescription-containing serum, IL-1β, and si RNA, Western blotting was used to determine the protein expressions of caveolin-1, Bax, Bcl-2, Fas, and caspase-3.RESULTS: The morphological score of the articular synovium in the model group was significantly higher than in the normal group(P < 0.01). The morphological score in the high-and mediumdose Gubi prescription groups was lower than in the model group(P < 0.05). Chondrocytes from the decoction-containing serum group had a lower expression of Bax(P < 0.05), and higher expressions of Bcl-2(P < 0.05) and caspase-3(P < 0.05) compared with the model group. Chondrocytes in the decoction-containing serum group had higher expressions of Bax and Bcl-2(P < 0.01) and lower expressions of caveolin-1 and

关 键 词：Osteoarthritis knee Caveolin 1 Phosphatidylinositol 3-kinase Superoxide dismutase MALONDIALDEHYDE Nitric oxide Interleukin-1beta Gubi prescription 

分 类 号：R285.5[医药卫生—中药学]