重组酶聚合酶扩增技术研究进展  

Research Progress in Recombinase Polymerase Amplification(RPA)

作　　者：施奕 徐昌平[2] 余蓓蓓[3] 卢亦愚[2] 梅玲玲[2] SHI Yi;XU Changping;YU Beibei;LU Yiyu;MEI Lingling(Institute of Life Science,Westlake Institute for Advanced Study,Hangzhou 310024,China;Institute of Microbiology,Zhejiang Center for Disease Control and Prevention,Hangzhou 310051,China;Shaw Affiliated Hospital,Zhejiang University,Hangzhou 310016,China)

机构地区：[1]西湖大学生命科学学院,杭州310024 [2]浙江省疾病预防控制中心微生物所,杭州310051 [3]浙江大学医学院附属邵逸夫医院,杭州310016

出　　处：《病毒学报》2020年第3期522-532,共11页Chinese Journal of Virology

基　　金：国家“十三五”科技重大专项艾滋病和病毒等重大传染病防治子项目(项目号:2018ZX10714002‐003‐007);浙江省医药卫生科技计划项目(项目号:2018274060、2018250999)。

摘　　要：重组酶聚合酶扩增(RPA)是一种近年来发展起来的等温扩增技术。不仅具有检测速度快,灵敏度高,特异性好等特点,而且由于该反应可以在体温条件下等温扩增,特别适用于实验室外的现场诊断与床边诊断,具有广泛的应用前景。本文对RPA技术的发展进行了回顾与综述,对RPA技术的扩增原理、反应机制、反应体系、引物探针设计的优化,以及在此基础上发展起来的RPA产物检测方法,如侧向流试纸、絮凝分析、实时荧光、电化学、化学发光、表面增强拉曼散射等诊断技术进行了全面阐述,同时分析了当前RPA技术发展中的热点问题,为该技术的深入研究和推广应用提供参考。Recombinase polymerase amplification(RPA)is an isothermal amplification technology developed in recent years. The RPA technology not only offer fast detection speed,highly sensitivity and specificity,it can also amplify at room temperature. It is especially suitable for in the field and bedside diagnosis outside the laboratory and has a broad application. Here,we summarizes the development of RPA technology,describes the principle of RPA,reaction mechanism,reaction system and optimization of reaction design of RPA technology. Field diagnosis methods have developed on this basis,such as lateral flow dipstick,flocculation analysis,real-time fluorescence analyses,electrochemistry,chemiluminescence and surface enhanced Raman scattering. We also evaluate the"hot topics"in current development of RPA technology. Our review will provide references materials for further research and application of RPA technology.

关 键 词：等温扩增 重组酶聚合酶扩增 检测 

分 类 号：R373[医药卫生—病原生物学]