女阴硬化性苔藓皮损黑素细胞和表皮厚度分析  

Analysis of melanocyte density and epidermal thickness in vulvar lichen sclerosus lesions

作　　者：高小曼[1] 李子媛 孙凯律 常建民[1] Gao Xiaoman;Li Ziyuan;Sun Kailyu;Chang Jianmin(Department of Dermatology,Beijing Hospital,National Center of Gerontology,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing 100730,China)

机构地区：[1]北京医院皮肤科,国家老年医学中心,中国医学科学院老年医学研究院,100730

出　　处：《中华皮肤科杂志》2020年第6期459-461,共3页Chinese Journal of Dermatology

基　　金：中国医学科学院医学与健康科技创新工程项目(2018-I2M-1-002)。

摘　　要：目的研究女阴硬化性苔藓(VLS)皮损黑素细胞密度及表皮厚度的变化。方法2018年6-12月于北京医院皮肤科收集15例成年VLS患者外阴皮损组织,根据病理表现皮损分为初期组7例、后期组8例,免疫荧光染色,通过图像分析软件计算黑素细胞密度,测量表皮全层和细胞层厚度。以中国医学科学院整形外科医院行外阴整形手术的15例成年女性的正常外阴皮肤作为对照组。结果初期组表皮黑素细胞密度为0.170±0.071,后期组为0.110±0.035,较对照组降低(0.275±0.036,F=36.426,P<0.001)。初期组表皮全层厚度为(203.682±137.997)μm,后期组为(150.020±70.914)μm,对照组为(194.030±82.996)μm,3组间差异无统计学意义(F=0.738,P=0.487)。表皮细胞层厚度初期组与对照组间差异无统计学意义(P=0.899),后期组低于对照组(P=0.003)。结论VLS初期及后期皮损表皮黑素细胞密度均降低;初期皮损表皮全层厚度及细胞层厚度均无明显改变,但后期细胞层厚度减少。Objective To analyze changes in melanocyte density and epidermal thickness in vulvar lichen sclerosus(VLS)lesions.Methods Vulvar skin tissues were collected from 15 adult female patients with VLS in Department of Dermatology,Beijing Hospital from June to December in 2018.According to pathological manifestations,15 skin lesions were divided into early-stage VLS group(n=7)and late-stage VLS group(n=8),and subjected to immunofluorescent staining.Then,density of melanocytes,and thickness of the whole epidermis and cell layers(from the bottom of the stratum corneum to the bottom of the basal layer)were calculated by using an image analysis software.Normal vulvar skin tissues were obtained from 15 healthy adult females after vulvar plastic surgery in Plastic Surgery Hospital,Chinese Academy of Medical Sciences,and served as control group.Results The density of melanocytes in the epidermis was significantly lower in the early-stage VLS group(0.170±0.071)and late-stage VLS group(0.110±0.035)than in the control group(0.275±0.036;F=36.426,P<0.001).There was no significant difference in the thickness of the whole epidermis among the early-stage VLS group(203.682±137.997μm),late-stage VLS group(150.020±70.914μm)and control group(194.030±82.996μm;F=0.738,P=0.487).The thickness of cell layers did not differ between the early-stage VLS group(154.603±121.984μm)and control group(176.974±80.296μm;P=0.899),but was significantly thinner in the late-stage VLS group(83.455±37.129μm)than in the control group(P=0.003).Conclusions The density of epidermal melanocytes decreased in the early-stage and late-stage VLS skin lesions.Compared with the normal skin tissues,the early-stage VLS lesions showed no significant changes in the thickness of the whole epidermis and cell layers,but the late-stage VLS lesions showed decreased thickness of cell layers.

关 键 词：外阴硬化性苔癣 黑素细胞 表皮 色素沉着不足 皮肤萎缩 

分 类 号：R758.6[医药卫生—皮肤病学与性病学]