亚麻油对宁乡猪不同组织长链脂肪酸谱的影响  

Effects of Linseed Oil on Long⁃Chain Fatty Acid Profile in Different Tissues of Ningxiang Pigs

作　　者：邢月腾[1] 郁元年 谢春艳 吴信[3] 肖定福[1] 杨志武 张彬[1] XING Yueteng;YU Yuannian;XIE Chunyan;WU Xin;XIAO Dingfu;YANG Zhiwu;ZHANG Bin(College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China;College of Resources and Environment,Hunan Agricultural University,Changsha 410128,China;Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;Ningxiang Animal Husbandry and Veterinary Bureau,Changsha 410600,China)

机构地区：[1]湖南农业大学动物科学技术学院,长沙410128 [2]湖南农业大学资源环境学院,长沙410128 [3]中国科学院亚热带农业生态研究所,长沙410125 [4]宁乡市畜牧兽医水产局,长沙410600

出　　处：《动物营养学报》2020年第6期2567-2574,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31872991,31902196);湖南省重大专项(2015NK1002);湖南省研究生科研创新项目(CX2016B277);宁乡市人民政府与湖南农业大学战略性合作项目(宁乡猪基础研究)。

摘　　要：本试验旨在研究亚麻油对宁乡猪背肌和3种不同脂肪组织中长链脂肪酸谱的影响,并探究脂肪酸生成的潜在机制。选取日龄相近、平均体重(43.21±0.64) kg的宁乡阉母猪30头,随机分为2组,每组5个重复,每个重复3头。对照组饲喂基础饲粮,试验组在基础饲粮中添加2%亚麻油,试验期56 d。结果显示:与对照组相比,饲粮中添加2%亚麻油后,宁乡猪背肌中花生一烯酸(C20∶1)、γ-亚麻酸(C18∶3n-6)、α-亚麻酸(C18∶3n-3)含量显著提高(P<0.05),花生四烯酸(C20∶4n-6)含量显著降低(P<0.05);饲粮中添加2%亚麻油对3个脂肪组织中脂肪酸含量的影响与背肌趋于一致,如能显著提高背脂、腹脂和肾周脂中亚油酸(C18∶2n-6c)、C18∶3n-6、C18∶3n-3、二十碳三烯酸(C20∶3n-6)含量(P<0.05),降低背脂和腹脂中油酸(C18∶1n-9c)(P<0.05)、C20∶4n-6(0.05≤P<0.10)及肾周脂中硬脂酸(C18∶0)含量(P<0.05);同时,饲粮中添加2%亚麻油还能显著降低3种脂肪组织中n-6/n-3多不饱和脂肪酸(PUFA)比值(P<0.05),使3种脂肪组织中平均n-6/n-3 PUFA比值降到5.92。此外,饲粮中添加2%亚麻油能显著下调背脂中乙酰辅酶A羧化酶α(ACCα)、γ-过氧化酶活化增生受体(PPARγ)和脂肪酸去饱和酶2(FADS2)基因的表达(P<0.05),对α-过氧化酶活化增生受体(PPARα)基因的表达也有抑制趋势(0.05≤P<0.10)。上述结果表明,饲粮中添加2%亚麻油能显著提高宁乡猪背肌、背脂、腹脂和肾周脂中C18∶3n-3含量,使3种脂肪组织中平均n-6/n-3 PUFA比值降至《中国居民膳食指南(2016)》推荐范围(4~6)内;这可能是通过调控细胞分化及抑制脂肪酸从头合成途径实现的。This experiment was conducted to study the linseed oil(LO)on fatty acid profile in longissimus dorsi muscle(LDM)and three different adipose tissues of Ningxiang pigs,and then investigate the underlying lipogenesis mechanisms.Thirty castrated female pigs with a similar age and average body weight of(43.21±0.64)kg were randomly allocated into 2 groups with 5 repetitions per group and 3 pigs per repetition.Pigs in control group were fed a basal diet,and those in experimental group were fed the basal diet supplemented with 2%LO for 56 days.The results showed that dietary supplemented with 2%LO significantly increased the con⁃tents of eicosenoic acid(C20∶1),γ⁃linolenic acid(C18∶3n⁃6)andα⁃linolenic acid(C18∶3n⁃3)in LDM(P<0.05),and significantly decreased the content of arachidonic acid(C20∶4n⁃6)in LDM compared with control group(P<0.05).The fatty acid contents in 3 adipose tissues influenced by 2%LO were analogous to those found in the LDM.The contents of linoleic acid(C18∶2n⁃6c),C18∶3n⁃6,C18∶3n⁃3 and epoxyeicosa⁃trienoic acid(C20∶3n⁃6)in dorsal subcutaneous adipose(DSA),abdominal subcutaneous adipose(ASA)and perirenal adipose(PA)were significantly increased(P<0.05)accompanied by a corresponding decrease in oleic acid(C18∶1n⁃9c)(P<0.05),C20∶4n⁃6(0.05≤P<0.10)in DSA and APA,as well as stearic acid(C18∶0)in PA(P<0.05).Meanwhile,dietary supplemented with 2%LO could significantly the ratio of n⁃6/n⁃3 polyunsaturated fatty acids(PUFA)in 3 adipose tissues(P<0.05),and make the average n⁃6/n⁃3 PUFA ratio reduce to 5.92.Furthermore,dietary supplemented with 2%LO could down⁃regulate the expression of ac⁃etyl coenzyme A carboxylaseα(ACCα),peroxisome proliferators⁃activated receptorγ(PPARγ),fatty acid desaturase 2(FADS2)genes in DSA(P<0.05),and had an inhibitory tendency on the expression of peroxi⁃some proliferators⁃activated receptorα(PPARα)gene in DSA(0.05≤P<0.10).These results indicate that dietary supplemented with 2%LO can significantly increase th

关 键 词：亚麻油 长链脂肪酸 脂代谢 宁乡猪 

分 类 号：S828.9[农业科学—畜牧学]