枸杞多糖对17℃负压环境下猪精子保存质量的影响  

Effect of Lycium Barbarum Polysaccharide on Pig Sperm Quality of Preservation under 17℃Negative Pressure Environment

作　　者：王硕 赵家辉 邹一诺 高明山 李雁冰[1] 黄大鹏[1] 魏国生[1] 李井春[1] WANG Shuo;ZHANG Jiahui;ZOU Yinuo;GAO Mingshan;LI Yanbing;HUANG Dapeng;WEI Guosheng;LI Jingchun(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Heilongjiang Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国畜牧杂志》2020年第5期95-99,共5页Chinese Journal of Animal Science

基　　金：黑龙江省自然科学基金面上项目(C2017053);黑龙江省博士后启动基金(LBH-Q18100);黑龙江八一农垦大学青年创新人才项目(CXRC2017004);黑龙江八一农垦大学博士启动基金(XYB-2016-04);黑龙江八一农垦大学研究生创新科研项目(YJSCX2019-Y28)。

摘　　要：为探究猪精液17℃负压保存条件下,不同浓度的枸杞多糖对猪精子保存质量的影响,本实验在Modena基础稀释液中分别添加0、0.5、1.0、1.5、2.0 mg/mL枸杞多糖,检测猪精子在此环境下5 d内的精子活率、活力、直线速度、路径速度、鞭打频率、畸形率等质量参数。结果表明:1.5 mg/mL组的精子活率、活力、直线速度、路径速度、鞭打频率和畸形率均显著优于其他组。由结果可知,Modena基础稀释液添加1.5 mg/mL枸杞多糖在17℃负压环境下更有利于猪精液保存。To investigate the effects of different concentrations of lycium barbarum polysaccharide on the survival time and quality of pig sperm under 17℃negative pressure,different concentrations of lycium barbarum polysaccharide were added into Modena basic diluent with 0,0.5,1.0,1.5,2.0 mg/mL,respectively.Live sperm rate,vigour,linear velocity,path to the speed,beat frequency,deformity rate were determined within 5 days.The results showed that live sperm rate,vigour,linear velocity,path to the speed,beat frequency,deformity rate of the 1.5 mg/mL group were significantly better than those of other groups.According to the results,the addition of 1.5 mg/mL of lycium barbarum polysaccharide to Modena base diluent was more beneficial to the preservation of pig semen under 17℃negative pressure.

关 键 词：枸杞多糖 猪精子 17℃ 负压 抗氧化 

分 类 号：S828.3[农业科学—畜牧学]