一株肉鸡源大肠杆菌的分离鉴定与致病性分析  

Identification and pathogenicity analysis of an Escherichia coli isolated from broilers

作　　者：丁树荣[1] 陆冰洋[1] 王彩先[1] 李婷婷[1] 刘华栋[1] 唐娟[1] 牛晋国[1] DING Shurong;LU Bingyang;WANG Caixian;LI Tingting;LIU Huadong;TANG Juan;NIU Jinguo(Institute of Animal Husbandry and Veterinary,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China)

机构地区：[1]山西省农业科学院畜牧兽医研究所,山西太原030032

出　　处：《家禽科学》2020年第5期45-48,共4页Poultry Science

基　　金：山西省农业科学院重点项目(YGG1609、YCX2018208、YCX2020YQ21)。

摘　　要：为了解规模化肉鸡养殖场致病性大肠杆菌的耐药性与致病性,并为规模化养殖场提供大肠杆菌病的防治参考。从山西省某规模化肉鸡养殖场采集病死鸡病料,进行大肠杆菌的分离培养、生化鉴定、药敏试验、致病性试验。结果表明:分离到1株血清型为O2的致病性大肠杆菌,分离菌对头孢类、喹诺酮类等8种药物耐药,对妥布霉素、阿米卡星高敏,对强力霉素、氧氟沙星中敏。结论:分离株肉鸡源大肠杆菌具有多重耐药性和较强的致病性,季节交替要加强鸡场饲养管理。In order to study the resistance and pathogenicity of E.coli in model feeding broilers farms,and to provide evidence for the prevention and treatment of E.coli in large-scale farms.E.coli form collected the samples form dead broilers in the broilers house in Shanxi were isolated,identified,drug sensitive test and pathogenicity analysis.The results showed that a stain pathogenic E.coli with O2 serotype was isolated.The isolated stain has multiple drug resistance to 8 kinds drugs including cephalosporins and quinolones,and highly sensitive to tobramycin and amikacin,and sensitive to doxycycline and ofloxacin.Conclusion:The isolated stain of E.coli from broiler has multiple drug resistance and highly pathogenicity.It is necessary to strengthen the feeding management of chicken farms in the alternate season.

关 键 词：肉鸡 大肠杆菌 分离鉴定 药敏试验 致病性分析 

分 类 号：S858.314.43[农业科学—临床兽医学]