铜绿假单胞菌注射液对直肠癌患者免疫功能的影响  

Effect of PA-MSHA on immune function in patients with rectal cancer

作　　者：张娟[1] 牛文博 周超熙[1] 王光林[1] 刘有强 丁源一[1] 安广权[1] ZHANG Juan;NIU Wen-bo;ZHOU Chao-xi;WANG Guang-lin;LIU You-qiang;DING Yuan-yi;AN Guang-quan(The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院,河北石家庄050011

出　　处：《中国病原生物学杂志》2020年第4期469-473,共5页Journal of Pathogen Biology

基　　金：河北省卫生厅重点科技研究计划(No.20160188)。

摘　　要：目的分析直肠癌患者术中腹腔应用铜绿假单胞菌注射液对其免疫功能的影响。方法选取河北医科大学第四医院结直肠癌围手术期患者200例,将患者分为实验组及对照患者,每组拟选择100例患者。实验组患者术中腹腔放置铜绿假单胞菌注射液10ml,术区及腹腔喷洒用药,于术后第1、3、7d采集静脉血行流式细胞学(FCM)检测相关指标。采用MTT比色法检测铜绿假单胞菌注射液对直肠癌细胞增殖抑制作用,采用流式细胞术分析分析细胞凋亡。结果术前、术后1d、术后3d、术后7d实验组CD3+T分别为67.12±11.03、56.98±11.52、63.51±12.06、73.56±13.39,CD4^+T分别为41.72±7.22、35.41±6.53、40.01±8.64、50.65±9.69,NK分别为12.51±4.62、10.16±4.45、11.65±5.16、15.51±4.68,CD4^+CD25^+T分别为5.16±1.08、4.58±0.92、4.22±1.02、3.96±1.16;术前、术后1d、术后3d、术后7d对照组CD3+^T分别为66.57±10.76、57.65±12.31、61.98±11.57、67.02±11.78,CD4^+T分别为40.36±8.59、34.96±6.79、38.54±7.82、40.51±9.12,NK分别为11.13±4.57、10.34±4.82、10.95±5.02、12.03±4.81,CD4^+CD25+^T分别为4.52±0.92、4.27±0.97、4.31±0.85、4.43±0.91。实验组和对照组肿瘤标记物CEA、CA199、CA724,肾功能BUN、CRE,肝功能ALT、AST和血常规WBC均在术前和术后数据差异均无统计学意义(P>0.05),而血常规RBC和HGB在术前和术后数据差异有统计学意义(P<0.05),实验组和对照组之间数据差异无统计学意义(P>0.05)。术后实验组和对照组患者均出现体温升高、恶心呕吐、口腔黏膜炎等不良反应,但无统计学意义。MTT比色法检测铜绿假单胞菌注射液对直肠癌细胞增殖抑制作用显示,加入铜绿假单胞菌注射液后各组48h后均产生不同的抑制效果。结论铜绿假单胞菌注射液对直肠癌细胞有抑制效果,无明显不良反应。Objectives The effect of PA-MSHA on immune function in patients with rectal cancer was analyzed.Methods Subjects were 200patients with colorectal cancer prior to undergoing surgery at the Fourth Hospital of Hebei Medical University.The patients were divided into an experimental group and a control group,with 100patients in each.The abdominal cavity of the experimental group was treated with a solution of 10ml of PA-MSHA during surgery,and the surgical site and the abdominal cavity were sprayed with that solution.On day 1,3,and 7after surgery,relevant indexes were determined using FCM.The inhibition of the proliferation of rectal cancer cells by PA-MSHA was determined using an MTT colorimetry assay,and apoptosis was analyzed using flow cytometry.Results In the experimental group,CD3^+T cells were 67.12±11.03before surgery,56.98±11.521day after surgery,63.51±12.063days after surgery,and 73.56±13.397days after surgery.CD4^+T cells were 41.72±7.22before surgery,35.41±6.531day af-ter surgery,40.01±8.643days after surgery,and 50.65±9.697days after surgery.NK cells were 12.51±4.62be-fore surgery,10.16±4.451day after surgery,11.65±5.163days after surgery,and 15.51±4.687days after surgery.CD4^+CD25^+T cells were 5.16±1.08before surgery,4.58±0.921day after surgery,4.22±1.023days after surgery,and 3.96±1.167days after surgery.In the control group,CD3^+T cells were 66.57±10.76before surgery,57.65±12.311day after surgery,61.98±11.573days after surgery,and 67.02±11.787days after surgery.CD4^+T cells were 40.36±8.59before surgery,34.96±6.791day after surgery,38.54±7.823days after surgery,and 40.51±9.127days after surgery.NK cells were 11.13±4.57before surgery,10.34±4.821day after surgery,10.95±5.023days after surgery,and 12.03±4.817days after surgery.CD4^+CD25^+T cells were 4.52±0.92before surgery,4.27±0.971day after surgery,4.31±0.853days after surgery,and 4.43±0.917days after surgery.Levels of CEA,CA199,CA724,BUN,CRE,ALT,and AST and the WBC count in the experimental group and control group did

关 键 词：铜绿假单胞菌注射液 直肠癌 抑制作用 

分 类 号：R378[医药卫生—病原生物学]