急性脑梗死患者合并感染病原菌的分布、氧化损伤及细胞因子水平检测分析  

Distribution of pathogens,oxidative damage and cytokine levels in patients with acute cerebral infarction infection

作　　者：陈菊华[1] 张元兴[1] 沈晓英[1] CHEN Ju-hua;ZHANG Yuan-xing;SHEN Xiao-ying(Department of Encephalology,Changzhou Hospital of Traditional Chinese Medicine,Jiangsu Province,Changzhou 213000,China)

机构地区：[1]江苏省常州市中医医院脑病科,江苏常州213000

出　　处：《中国病原生物学杂志》2020年第4期474-476,共3页Journal of Pathogen Biology

基　　金：江苏省中医药局科技项目(No.LZ13139)。

摘　　要：目的检测和分析急性脑梗死患者合并感染病原菌的分布情况、氧化损伤程度及细胞因子水平变化。方法随机选取本院2014年3月-2018年3月收治的急性脑梗死患者160例。其中并发感染患者80例为观察组,未并发感染80例为对照组。对观察组患者取样作病原菌的分离鉴定;抽取两组患者的静脉血,分离血清,采用酶联免疫吸附法(ELISA)检测总抗氧化能力(TAC)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、细胞间黏附分子-1(ICAM-1)、转化生长因子-β1(TGF-β1)及内皮素(ET-1)水平,并进行比较分析。结果80例急性脑梗死合并感染患者共检出病原菌87株。其中革兰阴性菌49株,占56.32%,以鲍曼不动杆菌(17株,19.54%)及铜绿假单胞菌(16株,18.39%)为主;革兰阳性菌38株,占43.68%,以酿脓链球菌(14株,16.09%)为主。与对照组比较,观察组患者血清中TAC、SOD及CAT水平显著下降,ICAM-1、TGF-β1及ET-1水显著升高(均P<0.05)。结论急性脑梗死患者合并感染的主要病原菌为革兰阴性菌;感染可导致患者氧化损伤加重,且相关细胞因子水平异常,临床治疗时应重视对上述指标的检测与干预。Objective To investigate the distribution of pathogenic bacteria,the degree of oxidative damage and the levels of cytokines in patients with acute cerebral infarction complicated with infection.Methods From March 2014to March 2018,160patients with acute cerebral infarction were randomly selected.Among them,80patients with complicated infection were in the observation group and 80patients without complicated infection were in the control group.The samples of patients in the observation group were collected for the isolation and identification of pathogenic bacteria.The venous blood of the two groups was extracted and the serum was separated.Total antioxidant capacity(TAC),superoxide dismutase(SOD),catalase(CAT),intercellular adhesion molecule-1(ICAM-1),transforming growth factor-β1(TGF-β1)and endothelin-1(ET-1)were measured by enzyme-linked immunosorbent assay(ELISA).Results A to-tal of 87pathogenic bacteria were detected in 80patients with acute cerebral infarction infection.56.32%were Gram-negative bacteria(49strains),mainly Acinetobacter baumannii(17strains,19.54%)and Pseudomonas aeruginosa(16 strains,18.39%).43.68%were Gram-positive bacteria(38strains),mainly Streptococcus pyogenes(14strains,16.09%).TAC,SOD,CAT,ICAM-1,TGF-β1and ET-1were(11.05±1.14)U/mL,(83.09±8.36)nU/mL,(41.45±4.23)nU/mL,(0.41±0.05)mg/L,(0.38±0.04)μg/mL and(51.25±5.23)pg/mL,respectively in the control group.The observation group was(8.26±0.87)U/mL,(71.34±7.25)nU/mL,(36.97±3.71)nU/mL,(0.65±0.09)mg/L,(0.49±0.07)μg/mL and(78.34±7.96)pg/mL respectively.The difference between the two groups was statisti-cally significant(t=17.401,9.497,7.122,20.850,12.203and 25.440,P<0.05).Conclusion Gram-negative bacteria are the main pathogens in patients with acute cerebral infarction complicated with infection.Infection can aggravate oxidative damage and abnormal levels of related cytokines in patients with acute cerebral infarction.The detection and in-tervention of these indicators should be emphasized in clinical treatment.

关 键 词：急性脑梗死 感染 病原菌 氧化损伤 细胞因子 

分 类 号：R378[医药卫生—病原生物学]