Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants  

作　　者：Yu Hao Wubei Zong Dongchang Zeng Jingluan Han Shuifu Chen Jianian Tang Zhe Zhao Xiaojuan Li Kun Ma Xianrong Xie Qinlong Zhu Yuanling Chen Xiucai Zhao Jingxin Guo Yao-Guang Liu 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Guangzhou 510642.China [2]College of Life Sciences,South China Agricultural University,Guangzhou 510642.China [3]Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642.China

出　　处：《Science China(Life Sciences)》2020年第6期933-935,共3页中国科学（生命科学英文版）

基　　金：grants from the National Natural Science Foundation of China (91435203 and 31991222);the Major Program of Guangdong Basic and Applied Basic Research (2019B030302006)。

摘　　要：Dear Editor,CRISPR(clustered regularly interspaced short palindromic repeats)/Cas genome editing is a powerful tool for introducing specific mutations in organisms including plants.The system is composed of a nuclease such as Cas9 or Cas12a and an engineered single-guide RNA(sgRNA)incorporating a target sequence(Li et al.,2019).A Cas9/sgRNA complex recognizes its target site in the genome,resulting in a mutation at that site.

关 键 词：RNA CRISPR Shortened snRNA promoters for efficient CRISPR/Cas-based multiplex genome editing in monocot plants 

分 类 号：Q943.2[生物学—植物学]